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《Industrial Microbiology》 1994-01
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Development and Characteristics of Glutamate Decarboxylasc Electrode

Fan Shongshan, Chen Shigen (Bicchemistry Department, Fudan University)  
Glutamate decarboxylase electrode was developed by immobilizing the gluta-mate decarboxylase on silicone rubber membrane of CO2-selective electrode using albumin and glutaraldehyde. The detective chamber was connected to the gluta-mate decarboxylase electrode system. This system exhibited good glutamic acid concentration response linearity (1.0×10-3.5M-l,0×10-1.5M with r=0.99 and CV 4.55%),high specificity (the influence of other amino acids was smaller than 5%), rapid response time (less than 2 min in dynamic assay, recovery time.5-10 min) and good storage and operation stability (30 days and 300 times, res-pectively).
【CateGory Index】: O646.5
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【References】
Chinese Journal Full-text Database 1 Hits
1 Wang Furong; Deng Bingbing(Department of Food Engineeing, TianJin Institute of Light industry,300222);Study and Characteristics of Glutamate Decarboxylase Electrode[J];FOOD AND FERMENTATION INDUSTRIES;1996-04
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Kong Jilie,He Hong,Deng Jiaqi (Department of Chemistry,Fudan University,Shanghai 200433);Comparative Studies on Enzyme and Microbial Sensors for Glutamic Acid[J];Chinese Journal of Analytical Chemistry;1993-03
2 Li Qingshan Ye Bangce Zhang Siliang Yu Juntang (Research institute of Biochemical Engineering East China University of Science and Technology, Shanghai 200237);Determination of Glutamate in Broth by Flow Injection Analysis Using an Immobilized Glutamate Oxidase Reactor[J];Chinese Journal of Biotechnology;1994-04
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