Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Modern Food Science and Technology》 2007-09
Add to Favorite Get Latest Update

Production and Evaluation of a Antibody Against Ractopamine

WANG Feng-xia ZHANG Yan HE Jin-xing WANG Wet XU Bei WANG Shuo (Tianjin Key Laboratory of Food Nutrition and Safety,College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)  
An indirect competitive enzyme-linked immunosorbent assay (ELISA) procedure was established to detect ractopamine employing a polyclonal antibody generated from RAC-linker-BSA and a coating antigen synthesized by method of mixed acid anhydride.The antibody against ractopamine showed high sensitivity with an IC_(50) of 0.9ng/mL and the detection limit (IC_(15)) was 0.1 ng/mL,which was lower than the data reported in literatures.The antibody with high specificity showed no cross-reactivity with clenbuterol,saibutamol,isoproterenol, terbutaline and isoxsuprine,while had the cross-reactivity of 7.5% with dobutamine.In addition,it was stable enough to be stored at 4℃for halfa year.This study laid a well foundation for the control of ractopamine by ELISA method.
【Fund】: 国家十一五科技支撑计划(No.2006BAD05A06)
【CateGory Index】: S859.84
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved