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《Modern Food Science and Technology》 2007-09
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Culture of Recombinant E.Coil Strain Producing Phenylalanine Ammonia Lyase(PAL)

CUI Jian-dong TAN Zhi-lei LI Fei JIA Shi-ru (Tianjin Key Laboratory of Industry Microbiology,Tianjin University of Science and Technology,Tianjin 300222,China)  
In order to improve the yield and activity of phenylalanine ammonia lyase (PAL) produced by recombinant E.coli strain,the culture conditions of recombinant E.coli strain carrying PAL gene of Rhodotorula toruloides was studied and optimized.The results showed that the optimal original glucose concentration,C/N ratio,the inoculation size and the initial pH value,fermentation time were 20g/L,7.86,10%, 7.5 and 18 h,respectively.It was also found that the PAL activity was significantly improved by adding 5g/L peptone and 5g/L yeast powder in fermentation medium.Under the optimized conditions,the PAL activity reached 70 U/g (DCW),which is 1.6 times higher than that under original fermentation conditions.
【CateGory Index】: TQ922
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