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《Sugarcane and Canesugar》 2001-01
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Preliminary Studies on QTL Mapping and Marker-assisted Selection in Sugarcane

K.K.Wu, Tim Wenslaff (Hawaii Agriculture Research Center. 99-193 Aiea Heights Drive, Aiea, Hi 96701, USA) Deng Haihua (Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316) K.K.Wu, Tim Wenslaff (Hawali Agriculture Research Center. 99-193 Aiea  
Quantitative trait loci (QTLs) controlling %pol, cane weight per plot, % fiber and %tasseling stalks in sugarcane were detected by interval mapping and point analysis (one-way ANOVA) using single-dose RFLP and RAPD marker information from a F1 population derived from La Purple (S. officinarum L. ) × Mol 5829 (S. robustum Brands and Jeswiet ex Grassel). Significant markers (P0. 01 in F-test or LOD 2) linked to Pol and Weight were selected for the further study on selection effects of marker-Assisted selection. For each trait, significant markers included both positive and negative ones. Point analysis identified more significant markers than interval mapping analysis, since it can detect unlinked markers associated with QTLs. The results indicated that the QTLs controlling both Pol and Weight were located in many linkage groups, while the QTLs controlling Tasseling concentrated on relatively fewer linkage groups. The results indicated that increasing the number of markers used for marker-assisted selection will result in higher mean trait value of the selected population, and lower risk of selecting lower trait value individuals in the mixed breeding population. The marker combination with each marker in its higher trait value marker class appeared to be best choice for multiple marker assisted selection, since it led to highest mean trait value of the selected population.
【CateGory Index】: S566.1
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