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《Acta Photonica Sinica》 2017-07
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The Design of Scanning Laser Optical Tomography Imaging System and Its Applications

XUE Cheng-zhi;LI Ya-min;LI Gui-ye;LI Ying-chao;HU Xue-juan;CHEN Ling-ling;Shenzhen Key Laboratory of Laser Engineering;Key Laboratory of Advanced Optical Precision Manufacturing Technology of Guangdong Higher Education Institutes,Shenzhen University;College of Electronic Science and Technology,Shenzhen University;College of Life Sciences and Oceanography,Shenzhen University;College of Optoelectronic Engineering,Shenzhen University;  
This paper presented a self-designed scanning laser optical tomography mesoscopic imaging system based on LabVIEW and explored its capabilities to image intact biological organs or tissues.The system is consisted of an aligned laser,a four dimension translation stage,a scanning mirror and a photomultiplier tube.The spatial resolution of the system is better than 20μm with an imaging field of1 cm.The system operation process and preliminary biomedical applications were demonstrated through the 3-D autofluorescence imaging of a mouse lung.Compared to the traditional biomedical optical imaging techniques,scanning laser optical tomography provides the advantages of high photon collection efficiency,large sample range and easy operation.
【Key Words】: Scanning laser optical tomography Fluorescence microscopy Mesoscopic size LabVIEW Biological organs Three-dimensional information Imaging systems
【Fund】: 国家自然基金(No.61505112);; 深圳市战略新兴产业发展专项资金(No.JCYJ20150525092941015);; 深圳市高端人才启动和深圳大学项目(No.201512);; 深圳市科技计划基础研究项目(No.JCYJ20150625101401072)资助~~
【CateGory Index】: TH74
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