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《Strait Pharmaceutical Journal》 2006-03
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The Extraction And Determination of Dichondra Repens Forst Polysaccharides

JIANG Jing-hua(Zhejian Dongyan City Pharmacy Corperation Co.Ltd,Dongyan 322100,chian)  
OBJECTIVE The comparison of Ultrasonic ware extraction in different temperatures(20,40,80℃) with water-bath extraction was proposed to obtain the optimal condition of the extraction of Dichondra repens Forst Polysaccharides.METHODS Using Ultrasonic methods with different temperatures as well as water-bath extraction,the Polysaccharides were obtained by water extraction and ethanol precipitation.Then use the method of Sevag to excise the protein.The determination was performed at wavelength 490nm with phenolsulfuric acid as a color reagent.RESULTS The temperatures were in proportion to the efficiency of extraction when the Ultrasonic methods were used.But on the whole,the method of water-bath extraction was more efficient.CONCLUSION Through comparing and analysing the efficiency of the extraction in different conditions,we can establish the substratum for the further resrarch of extraction,analysis and pharmacology of the Dichondra repens Forst Polysaccharides.
【CateGory Index】: R284
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