Studies of the Optimum Extraction Process of Alkannin from Arnebia Euchroma(Royel) Johnst in Xinjiang
ZHOU Hua-geng;LIU Xiao-hua;FU Zhi-jun;Department of Pharmaceutics of Traditional Chinese medicine,China Pharmaceutical University;
OBJECTIVE To optimize the extraction of alkannin from Arnebia euchroma( Royel) Johnst by orthogonal design. METHODS Referring to the content of alkannin,HPLC was employed for the content determination with Hedera ODS-2( 4. 6mm × 250 mm,5μm) and the mobile phase of methanol-0. 05% formic acid solution( 80∶ 20) at detection wavelength of 516 nm. The column temperature was 30℃ and flow rate was 1. 0m L·min- 1. Factors of temperature、times amount of 95% ethanol and extraction time were studied. RESULTS Alkannin had linear in the range between 2. 5 ~ 40. 0μg·m L- 1,r = 0. 9999; The recovery was 99. 96%. The optimal process of extraction was as follows: the extraction time was 4h,adding 10 times 95% ethanol,at 50℃. CONCLUSION The extract process is reasonable and practicable,which can be used as basis for the extraction process of alkannin.