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《Strait Pharmaceutical Journal》 2018-08
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Development of an Immunochromatographic Assay for Screening of Oxaprozin as Illegal Adulterant in Foods

HUANG Wan-qiong;ZHANG Min-ying;CHEN Hua-Long;LIU Meng;JIANG Li-Jun;GUO Jie-Biao;Shaoguan College;Shaoguan Institute for Food and Drug Control;  
OBJECTIVE An immunochromatographic assay( ICA) for screening oxaprozin as illicit adulterant in food was developed in this research. METHODS Artificial antigens were prepared by conjugating oxaprozin to OVA and BSA using modified active ester mechanism. Antibodies against oxaprozin were raised from rabbits by immunizing with BSA-oxaprozin conjugate. RESULTS Results indicated that the IC_(50) value for oxaprozin was 13. 2 ng·mL~(-1) in the ELISA. The cross-reactivities for 8 associate drugs were lower than 0. 1%. A consequent ICA showed detection limit of 2. 0 ng·mL~(-1) for oxaprozin. The current ICA showed good agreements with HPLC in detecting oxaprozin in 30 food samples from local market. CONCLUSION The ICA is suitable for screening illegal adulterant of oxaprozin in foodstuffs due to highly sensitive and specific.
【Fund】: 广东省食品药品检验检测技术创新项目(2015ZX09);; 广东大学生科技创新培育专项资金(攀登计划专项资金pdjh2017b0457);; 全国大学生创新训练计划立项(201410576009)
【CateGory Index】: R155.5
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