Determination the Content of Arginine in Breviscapine for Injection by HPLC
WANG Xian-ran;WANG Hua;LI Zhou;Department of Pharmacy,The First Affiliated Hospital,University of South China;
OBJECTIVE To establish a HPLC for determination of Arginine in Breviscapine for Injection. METHODS Agilent TC-C_(18)( 4. 6 mm × 250 mm,5μm) was used as chromatographic column,50 mmol·L~(-1) potassium dihydrogen phosphate ∶ acetonitrile( 95 ∶ 5) as mobile phase,and the detection wavelength was at206 nm. RESULTS The liner correlation observed was in the rang of 200μg · mL~(-1) to 1500μg · mL~(-1),r =0. 9996,The average recovery was 99. 93%,RSD was 0. 9%,n = 9. The average content of Arginine in Breviscapine for Injection was about 11. 79 mg by using this method. CONCLUSION This method is good specificity,repeatability,rapid and accurate,and can be used as part of the argrinine in Breviscapine for Injection,the method for the determination of arginine content.
【CateGory Index】： R284.1;O657.72