Content Determination of Chloroogenic Acid in Yinbing Gel by HPLC
ZHENG Wang-sheng;JIANG Yu-jia;WANG Ping;College of pharmacy Zhejiang University of Technology;Hainan Asia Pharmaceutical Co.,Ltd;
OBJECTIVE To establish a HPLC method for the content determination of chlorogenic acid in Yinbing Gel. METHODS Agilent C_(18) column( 5μm,250 mm × 4. 6 mm) was used with a mobile phase of acetonitrile-1. 6% acetic acid solution( 11∶ 89),The sample was determined at the wavelength of 327 nm and the temperature of column was 25℃. The flow rate was 1. 0 mL·min~(-1) and sample size was 20μL each time precisely. RESULTS Chlorogenic acid had a good linear relationship within 2. 02 ~ 101. 00μg·mL~(-1)( r = 0. 9994),the average recovery was 98. 14%,RSD was 1. 42%. CONCLUSION This method is simple,rapid,accurate and reliable,which can be used for the quality control of Yinbing Gel.
【CateGory Index】： R286.0;O657.72