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《Strait Pharmaceutical Journal》 2018-08
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Content Determination of Paeoniflorin in ChangMaiLe Capsule by HPLC

WENG Yi-ling;CHEN Song-wang;LI Huang;The second Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine;Fujian medical equipment and pharmaceutical packaging materials inspection institute;College of Pharmacy,Fujian University of Traditional Chinese Medicine;  
OBJECTIVE To research and establish a method for the determination of paeoniflorin in ChangMai Le capsule by HPLC. METHODS The mobile phase was consisted of the acetonitrile-0. 1% phosphoric acid solution( 18∶ 82). The chromatographic column was Diamonsil C_(18)( 2)( 5μm,250 × 4. 6 mm). The column temperature was 30℃. The injection volume was 10μL. Detection was performed at 230 nm with eluent flow rate of 1 mL·min~(-1). RESULTS According to the above experimental methods,the paeoniflorin have good linear relationship with the peak area in the range of 12. 11μg·mL~(-1)-151. 4μg·mL~(-1)( r = 0. 9997). While the average recovery of paeoniflorin was 97. 40%,and the RSD was 1. 69%( n = 6). CONCLUSION The method has an accurate result and can be used as a method for detecting the content of paeoniflorin in ChangMaiLe capsule. And used to control the quality of ChangMaiLe capsule.
【CateGory Index】: R286;O657.72
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