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《Journal of Hebei University(Natural Science Edition)》 2006-01
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Construction of Targeting DNA Vaccine pcDNA3/MDC-VP1 and its Immunological Effect on Mice

WANG Xiao-ling,WU Shu-hui,GAO Zhi-yun,WANG Yong-xiang (Department of Pathogenic Biology,Hebei Medical University,Shijiazhuang 050017,China)  
Macrophage-derived chemokine(MDC) gene fragment was amplified by RT-PCR,and linked to Coxsackievirus B3(CVB3) VP1 gene to construct an eukaryotic expressing plasmid pcDNA3/MDC-VP1 by a DNA sequence encoding a flexible polypeptide(10 amino acids).The plasmid was inoculated to mice as a vaccine.After the third inoculation,the mean neutralizing antibody titer in pcDNA3/MDC-VP1 group was higher than that of pcDNA3/VP1,and the virus titer of blood in pcDNA3/MDC-VP1 group was lower than that of pcDNA3/VP1.After 10 LD-(50) CVB3 challenge,the survival rate of pcDNA3/MDC-VP1 group was 50%,Kaplan-Meier test was used to compare differences of survival curves,the results showed that the survive state of pcDNA3/MDC-VP1 group is better than that of other groups(P0.05).
【Fund】: 河北省自然科学基金资助项目(C2004000631)
【CateGory Index】: R392
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