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《Journal of Hebei University(Natural Science Edition)》 2006-01
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Protoplasts Preparation,Regeneration and Mutagenesis of Streptomyces tsukubaensis

WANG Xiao-lei,GAO Hui-ying,JIANG Qin,LIU Gang-san,ZHANG Hua,HE Jian-gong(National Microbial Medicine Engineering & Research Center,New Drug Research and Development Co.,Ltd.of North China Pharmaceutical Corporation, Shijiazhuang 050015,China)  
Tacrolimus,a novel immunosuppressant isolated from Streptomyces tsukubaensis, has an impotant value of medical application.To screen high tacrolimus-producing mutants in S.tsukubaensis,the methods of protoplast preparation,regeneration and mutagenesis were established.Thereafter,the protoplasts of the original strain were treated by UV.Single colonies,which were formed by the regeneration of the UV-treated protoplasts,were selected to check their tacrolimus yield respectively.A high-yield mutant was obtained.The production of tacrolimus was increased 51.4% in comparison with the original strain.The result indicated that treating the protoplast of S.tsukubaensis with UV was a feasible and effective way to improve the production of tacrolimus.
【Fund】: 国家科技部创新药物筛选技术平台研究资助项目(2002AA2AZ343D)
【CateGory Index】: TQ465
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