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《Journal of Hebei University(Natural Science Edition)》 2006-01
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Study of Interaction Between Specific Toxin Produced by Exserohilum Turcicum and Membrane Protein

WANG Shao-xin~1,LIU Ying-chao~1,LI Zheng-ping~2,DONG Jin-gao~1(1.Mycotoxin Laboratory,Agricultural University of Hebei,Baoding 071001,China;2.College of Chemistry & Environmental Science,Hebei University,Baoding 071002,China)  
The effect of the toxin on protoplasmic membrane protein was studied by the method of fluorescence,using 8-anilino-1-naphthalene-sulfonic acid(ANS) as probe and taking the mesophyll protoplasts from maize leaves as the research system.After the liquid of protoplasts labeled ANS was injected with different toxin conc.,dosage-effect relationship existed because of the ANS fluorescence intensity increasing obviously,which indicated the toxin had some kinds of effects on receptor of ANS.Two types of reagents were employed to prove the proteic characteristic of ANS receptor,the result of which demonstrated the proteic characteristic of the toxin.It was concluded that the first action site of the specific toxin might be on the outside of protoplasmic membrane,moreover,the toxin receptor has the proteic characteristic and the combination between toxin and its receptor has saturation activity.
【Fund】: 河北省自然科学基金资助项目(302318);; 国家教育部骨干教师计划资助项目;; 河北农业大学9816科技重点项目
【CateGory Index】: S435.131
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