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《Journal of Hebei University(Natural Science Edition)》 2006-01
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Genotyping of Pseudomonas aeruginosa by Using Randomly Amplified Polymorphic DNA

LIU Jin-lu,WANG Lei,LI Cai-qing (The First Affiliated Hospital,Hebei North University,Zhangjiakou 075000,China)  
To find out the infection performance of Pseudomonas aeruginosa(PA) in clinical practice of all sorts,and set up a stable clinical monitoring system of cross infection PA strains were grouped by RAPD typing method and microdilution drug-senstive test typing.All the 27 PA specimen collected were divided into 6 types according to different degrees of antibiotics drug-resistance,of which Type Ⅰmaking up 44.5%;TypeⅡ making up 18.5%;Type Ⅲ amounting to 7.4%;Type Ⅳ amounting to 7.4%;Type Ⅴ taking up 11.1%;Type Ⅵ amounting to 11.1%.2 RAPD typing fell into nine different genotypes.The Genotype A amounting to 51.9%;Genotype B,7.4%;Genotype C 7.4%;Genotype D,7.4%;Genotype E,7.4%;Genotype F,(3.7%);Genotype G,3.7%;Genotype H,7.4%;Genotype I,3.7%.PA strains of Genotype A and Drug-Fast Spectrum Ⅰ were the cause of infection in ICU ward,and other strains were not relevant to the infection.The sensitivity of RAPD typing,identification,and reliability obviously prevails over drug-resistance spectrum typing,so it has considerable significance in the stuy of molecular epidemiology.PA strains were highly resistant to Piperacillin,Cefuroxime and Cefuroxime;comparatively sensitive to Carbostyril antibiotics;resistant to amimo sugar antibiotics in some degree and least resistant to Imipenem.
【Fund】: 河北省科技攻关指导项目(012761169)
【CateGory Index】: R378
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1 YE Ming Liang 1, YUAN Zhu Xin 1, LI Xiao Di 1, WANG Quan Li 2 (1.Ji′nan Military General Hospital, Jinan 250031,China ; 2.Institute of Field Transfusion Medicine,Academy of Military Medical Sciences, Beijing 100850,China) [;Optimization of method for extraction of template of bacteria for randomly amplified polymorphic DNA[J];Bulletin of The Academy of Military Medical Sciences;2001-02
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1 GUO Ai-ling et al(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070);Establishment of RAPD Identification Method for Shigella[J];Journal of Anhui Agricultural Sciences;2008-11
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5 YE Ming_Liang 1, WU Hai_Huan 1, WANG Quan_Li 2 (1.Jinan Military General Hospital, Jinan 250031, China; 2. Institute of Field Transfusion, Academy of Military Medical Sciences, Beijing 100850, China);Genotyping of Pseudomonas aeruginosa by randomly amplified polymorphic DNA analysis and its application[J];Bulletin of The Academy of Military Medical Sciences;2001-04
6 YE Ming-Liang 1, XIAO Peng-Yun 1, WANG Quan-Li 2 (1. General Hospital, Jinan Military Command, Jinan 250031, China; 2. Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China);Genotyping of Enterobacter aerogenes by randomly amplified polymorphic DNA technique[J];Bulletin of The Academy of Military(Medical Sciences);2004-03
7 YE Mingliang,WU Haihuan,HOU Xiaoqi,LU Bo(Jinan Military General Hospital,Jinan 250031,China);Genotyping of Acinetobacter baumannii by randomly amplified polymorphic DNA technique[J];Journal of Clinical Laboratory Science;2005-03
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