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《Journal of Hebei University(Natural Science Edition)》 2007-01
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Cloning and Expression of Rabbit Brain Tissue Factor Gene in Escherichia coli

LI Dai-zong,NI Zhi-hua,ZHAO Xiao-yu(College of Life Sciences,Hebei University,Baoding 071002,China)  
The tissue factor gene was amplified by the method of RT-PCR from the template of total RNA extracted from fresh rabbit brain,then cloned into the pMD19-T vector and the DNA sequence was determined.The correct identified recombinant was used as a template for amplification of TF gene by PCR and the product was inserted into the fusion expression vector pMAL-c2X.SDS-PAGE and Western blotting showed that the recombinant pMAL-c2X-TF had expressed a soluble fusion protein in E.coli DH5α.The fusion protein was purified by affinity chromatography,and the TF protein obtained after Factor Ⅹa cut had coagulant activity.
【CateGory Index】: Q78
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