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《Journal of Hebei University(Natural Science Edition)》 2008-05
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To Optimize Spore Formation Conditions of Antagonistic Strain 1-12 of Bacillus megaterium Against Colletoichum gloeosporioides

WANG Qian1,LI Shu-na1,ZHU Bao-cheng1,LI Lu-bin2,ZHUANG Cai-yun2,PENG Zhen-hua2(1.College of Life Sciences,Agriculture University of Hebei,Baoding 071001,China;2.Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091, China)  
In order to improve the spore formation rate and spore quantity of antagonistic strain 1-12 of Bacillus megaterium against Colletoichum gloeosporioides,the main factors influencing spore formation were investigated using shake-flask fermentation method.Through single factor experiment and orthogonal experiment,the optimal shaking flask fermentation conditions were determined as follow: medium composed(mass fraction) of 1% corn flour,1% soybean peptone,0.1%CaCl2·2H2O,0.05% MnSO4·H2O.Initial pH 6.0,incubation time 20 h and flask coutent 30 mL / 250 mL,8% inoculum size,fermentation temperature 30 ℃,rotating speed 200 r/min.Under this optimum conditions,1.92×109 mL-1 were obtained, and the spore formation rate achieved 95%.
【Fund】: 引进国际先进林业科学技术创新项目“花卉新品种创制技术引进与创新”(2006-4-C08)
【CateGory Index】: S436.8;S476
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