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《Journal of Hebei University(Natural Science Edition)》 2008-05
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To Optimize Spore Formation Conditions of Antagonistic Strain 1-12 of Bacillus megaterium Against Colletoichum gloeosporioides

WANG Qian1,LI Shu-na1,ZHU Bao-cheng1,LI Lu-bin2,ZHUANG Cai-yun2,PENG Zhen-hua2(1.College of Life Sciences,Agriculture University of Hebei,Baoding 071001,China;2.Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091, China)  
In order to improve the spore formation rate and spore quantity of antagonistic strain 1-12 of Bacillus megaterium against Colletoichum gloeosporioides,the main factors influencing spore formation were investigated using shake-flask fermentation method.Through single factor experiment and orthogonal experiment,the optimal shaking flask fermentation conditions were determined as follow: medium composed(mass fraction) of 1% corn flour,1% soybean peptone,0.1%CaCl2·2H2O,0.05% MnSO4·H2O.Initial pH 6.0,incubation time 20 h and flask coutent 30 mL / 250 mL,8% inoculum size,fermentation temperature 30 ℃,rotating speed 200 r/min.Under this optimum conditions,1.92×109 mL-1 were obtained, and the spore formation rate achieved 95%.
【Fund】: 引进国际先进林业科学技术创新项目“花卉新品种创制技术引进与创新”(2006-4-C08)
【CateGory Index】: S436.8;S476
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【References】
Chinese Journal Full-text Database 2 Hits
1 Yang Shengyuan1 Wei Jin2 Li Yun1(1Biology Department of Hanshan Normal University,Chaozhou 521041;2Library of Hanshan Normal University,Chaozhou 521041);Comparative Analysis on the Biologic Characters of Two Endophytic Bacillus megaterium[J];Biotechnology Bulletin;2010-03
2 SI Lu-jun1,2,GUO Qing-yuan1,WANG Xiao-ming2(1.College of Agriculture,Xinjiang Agricultural University,Urumqi 830052; 2.Institute of Crop Science/The National Key Facilities for Crop Genetic Resources and Improvement, Chinese Academy of Agricultural Sciences,Beijing 100081,China);Identification of Causal Agent of Maize Bacterial Leaf Spot in Dongyang of Zhejiang Province[J];Journal of Maize Sciences;2011-01
【Citations】
Chinese Journal Full-text Database 1 Hits
1 XU Shi-rong, CHEN Xiang, WU Yun-peng(College of Bioengineering, Chongqing University, Chongqing 400044,China);Application of the Mechanism of Sporulation in Production of Pharmaceutical Probiotics[J];Journal of Food Science and Biotechnology;2007-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 QIN Jian-bin,WEI Cui-hua,LIN Dan-qing (Agricultural Science Research Institute of Fuzhou,Fuzhou 350019,China);Control Effect of Different Chemicals on Soft Rot of Cymbidium hybridum[J];Shandong Agricultural Sciences;2010-09
2 Wang Chundi et al.(Root and Tuber Crop Research Institute,Yunnan Normal University,Kunming 650092,China);Overview of Research on Mating Type of Phytophthora infestans on Potatoes[J];Anhui Agricultural Science Bulletin;2010-13
3 Qi Rende et al (Institute of Plant Protection, AAAS, Hefei 230031);Studies on Pathogenicity of Metalaxyl-resistant Isolation of Phytophthora Caprice to Capsicum[J];Journal of Anhui Agricultural Sciences;2001-02
4 QI Ren-de et al (Institute of Plant Protection,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031);Inheritance of Biological Characteristics of Phytophthora capsici Metalaxyl-resistant mutants in Asexual Reproduction[J];Journal of Anhui Agricultural Sciences;2008-13
5 LIU Zhi-cheng et al (College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642);tudy on Pathogen and Control of the Pseudobulb Rot of Cymbidium sinense[J];Journal of Anhui Agricultural Sciences;2008-25
6 MU Yan-kui1 HE Juan2 ZHOU Zheng1 ZHOU Wei1 WU Tao1(1 Hebei Institute of Food Quality Supervistion Inspection and Research,Shijiazhuang Hebei 050051;2 Hebei Zhongrun Pharmaceutical Company Limited);The Fermentation Process and Quality Control of Vegetable Microecology Preparation[J];Modern Agricultural Sciences and Technology;2010-16
7 CHEN Fang-xin,GAO Zhi mou,QI Yong xia,MA Guo shen (Department of Plant Protection,Anhui Agricultural University, Hefei 230036);On Identification and Biological Characters of the Causal Organism of Cotton Boll Blight in Anhui[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-03
8 LI Ping1,QI Ren-de2,YANG Guang-hong1,XU Zhou-dian1,GAO Zhi-mou1(1.College of Plant Protection,Anhui Agricultural University,Hefei 230036; 2.Institute of Plant Protection,Anhui Academy of Agricultural Sciences,Hefei 230031);Preliminary studies on relationship between mating type and pathogenicity of Phytophthora capsici[J];Journal of Anhui Agricultural University;2011-03
9 Gao Zhimou Zheng Xiaobo Lu Jiayun (The Key Lab of Mornitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture,Department of Plant Protection,Nanjing Agricultural University,Nanjing 210095);Effects of Light,Humidity and Medium on Growth Rate of Phytophthora boehmeriae[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1998-02
10 ZHANG Da-hao TAN Tian-wei WANG Bing-wu (College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Response surface analysis of lipase production by Penicillium camembertii Thom[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2006-02
China Proceedings of conference Full-text Database 1 Hits
1 ;Isolation,Identification and Pathotype Distribution of Phytophthora sojae from Anhui Province[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HUANG Jian-fei et al(College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090);Screening,Identification and Antibiotic Effect of a Strain of Bacillus[J];Journal of Anhui Agricultural Sciences;2008-06
2 DENG Mo-yuan,WANG Bo-chu,YANG Zai-chang(College of Bioengineering,Chongqing University,Chongqing 400044,China);The Application of Techniques of Molecular Biology in Classification and Identification of Endophytes[J];Amino Acids & Biotic Resources;2006-03
3 LI Xinrong1,ZHANG Yuanming2,ZHAO Yunge3(1.Cold and Arid Regions Environmental and Engineering Research Institute,CAS,Lanzhou 730000,China;2.Xinjiang Institute of Ecology and Geography,CAS,Urumuq 830011,China;3.Institute of Soil and Water Conservation,CAS and Ministry of Water Resources,Yangling 712100,China);A Study of Biological Soil Crusts:Recent Development,Trend and Prospect[J];Advances in Earth Science;2009-01
4 LIU Qi-li1,ZHANG Jian-xin2,LI Jian-min1,SONG Pan1,CAO Jin-hai1(1.Department of Plant Protection,Henan Institute of Science and Technology,Xinxiang 453003,China;2.College of Life Science,Henan Normal University,Xinxiang 453007,China);Identification of pathogen of faber cymbidium anthracnose from Cailan Mountain Areas in Luanchuan county and screening of fungistats extracted from plants[J];Guangdong Agricultural Sciences;2009-09
5 YU Shu chi 1, ZHANG Li ping 1, WANG Li an 2 (1.School of life Sciences, Hebei University,Baoding 071002, China; 2. School of life sciences, Hebei Normal University,Shijiazhuang 050016,China);A Procedure of Research on the Antagonistic Bacterium as a Means of Biocontrol[J];Journal of Hebei Agricultural Sciences;2004-01
6 LONG Su\+\{1,2\}\ LI Fa\|feng\+2\ CHEN Ming\+2\ LIN Min\+2 (1 Hunan Medical University,Changsha Hunan prov.410078; 2\^Institute for Application of Atomic Energy,CSSA,Beijing\ 100094);THE ENHANCED EFFECT OF CO\|CULTURE ON NITROGEN\|FIXING ACTIVITY OF B.sphaerium AND B.megaterium[J];(拉丁文)ACTA AGRICULTURAE NUCLEATAE SINICA;2000-06
7 JIN Hong-wei,LIU Gui-lan,WANG Geng,FANG Bai-shan(Laboratory of Industrial Biotechnology of Fujian Province University,Huaqiao University,Quanzhou 362021,China);Isolation,Identification and Phylogenetic Analysis of a Collagenase Strain from Bacillus megaterium[J];Journal of Huaqiao University(Natural Science);2008-03
8 LIU Wen-Hai1,DENG Xian-Yu1,2,XIANG Yan-Ci1,QIU Shan-Hong3(1.School of Life Sciences,Hunan University of Science and Technology,Xiangtan,411201;2.Cycle Eco-Agriculture Company Ltd.,Xiangtan Couty,Xiangtan,411200;3.No.3 Middle School of Huaihua City,Huaihua,418000);BACILLUS MEGATERIUM,AN EFFICIENT BIODEGRADER TO METHAMIDOPHOS:ISOLATION AND MOLECULAR IDENTIFICATION[J];Oceanologia Et Limnologia Sinica;2009-02
9 CHENG Liang1,2,YOU Chun-ping1,2*,XIAO Ai-ping1(1.Zhongkai Agrotechnical College, Guangzhou 510225, China; 2.College of Agronomy, JAU, Nanchang 330045, China);Advance in the Study on Antagonistic Bacteria[J];Acta Agriculturae Universitis Jiangxiensis;2003-05
10 WU Nan, WANG Hongling, LIANG Shaoming, NIE Huali& ZHANG Yuanming Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;Temporal-spatial dynamics of distribution patterns of microorganism relating to biological soil crusts in the Gurbantunggut Desert[J];科学通报(英文版);2006-S1
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 YANG Sheng-yuan1,WEI Jin2,LI Yun1,YAO Hong1,HUANG Yue-dan1(1.Department of Biology,Hanshan Normal University,Chaozhou 521041,China ;2.Library of Hanshan Normal University,Chaozhou 521041,China);Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances[J];Food Science;2010-21
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Kuang Qun, Sun Mei, Shi Dalin;Study of Culturing Conditions of Clostridiun Butyricum[J];Feed Industry;2005-10
2 Wu Ye 1 Liu Zhongjian 2 Luo Huanliang 1 Chen Weiyuan 2 Li Sen 2 Zhang Jinning 1 (1 Foresty College, South China Agr.Univ. Guangzhou ,510642; 2 Wutong Shan Nuseries, Shenzhen);STUDY ON THE ORCHID DISEASE Stagonospora curtisii[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1997-03
3 Zhang Kaiming Li Yidong Zheng Fucong Huang Qingchun (Plant Protection Research Institute,SCATC);PHYTOPHTHORA SPECIES ON RUBBER TREES IN CHINA[J];Chinese Journal of Tropical Crops;1990-02
4 LI Ye ** ZHANG Xiao-Ping ZHANG Ke-Qiang LI Jun-Xing SUN Wen-Jun(College of Resource & Environment Science of Sichuan Agricultural University, Yaan 625014)1 (Agro-Environmental Protection Institute of MOA ,Tianjin 300191)2 (Tianjin Jinnan National Agriculture Science & Technology Zone, Tianjin 300351)3;Optimization of Conditions on Submerged Fermentation of Bacillus cereus[J];Microbiology;2005-02
5 Zhang Kaiming Li Yidong Zheng Fucong(Plant Protection Research Institute,SCATC);IDENTIFICATION AND MATING TYPE OF PHYTOPHTHORA SPECIES ON CITRUS TREES IN HAINAN AND ZHANJIANG[J];Acta Phytopathologica Sinica;1993-02
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