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《Chemistry & Bioengineering》 2013-06
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Study on 2,3-Butanediol Production by Serratia Marcescens G1 Using Corn Steep Liquor Powder and Ammonium Dibasic Phosphate

HAO Ying-li1,ZHANG Liao-yuan1,SUN Jian-an1,WEI Dong-zhi1, ZHOU Wen-yu1,SHEN Ya-ling1,ZHU Jia-wen2(1.New World Institute of Biotechnology,State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology,Shanghai 200237,China; 2.Chemical Engineering Research Center,East China University of Science and Technology,Shanghai 200237,China)  
Effects of several industrial nitrogen sources on biomass and 2,3-butanediol production for Serratia marcescens G1 were studied.The results showed that corn steep liquor powder(CSLP) was the optimum industrial nitrogen source.Based on the previous experiments,the optimum concentrations of CSLP and ammonium dibasic phosphate[(NH4)2HPO4] were optimized by PB design and response surface methodology(RSM).The optimal medium was determined as follows:sucrose 90 g·L-1,CSLP 20.32 g·L-1,(NH4)2HPO4 7.21 g·L-1,NaAc 4 g·L-1,sodium citrate 14 g·L-1,MgSO4 0.5 g·L-1,FeSO4 0.02 g·L-1,MnSO4 0.01 g·L-1.The optimal medium was used to perform shaking fermentation and fed-batch fermentation experiments.Ultimately,the strain converted 90 g·L-1 sucrose to 43.06 g·L-1 2,3-butanediol in shaking flasks,in fed-batch fermentation,a 2,3-butanediol level of 128.28 g·L-1 was obtained,and the transformation rate and yield of 2,3-butanediol achieved to 0.48 g·g-1 and 2.67 g·L-1·h-1,respectively.It provides the foundation for industrial production that using CSLP and(NH4)2HPO4 as nitrogen sources with higher production of 2,3-butanediol,lower cost of medium.
【Fund】: 国家863计划资助项目(2006AA02Z243);; 国家重点实验室专项经费资助项目(2060204);; 上海市重点学科建设资助项目(B505)
【CateGory Index】: TQ223.162
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1 YANG Yun-long1,ZHANG Liao-yuan1,SUN Jian-an1,WEI Dong-zhi1,ZHOU Wen-yu1,SHEN Ya-ling1,ZHU Jia-wen2 (1.New World Institute of Biotechnology,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China; 2. Chemical Engineering Research Center,East China University of Science and Technology,Shanghai 200237,China);Study on 2,3-Butanediol Production by Serratia Marcescens Using Sucrose and Ammonium Citrate[J];Chemistry & Bioengineering;2009-09
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