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《Journal of Hebei Agricultural Sciences》 2017-02
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Study on Improvement of Callus Regeneration Frequency in Maize

LI Hai-xia;SUN Jin-hua;XING Guo-zhen;WANG Ai-wu;He'nan Agricultural University;  
In order to improve the regeneration rate on maize callus, different 2,4-D concentration including0, 0.5, 1.0 and 1.5 mg/L were used to pretreatment callus media of N6 +700 mg/L proline +500 mg/L casein hydrolysate+30 g/L sucrose. The regenerate rate of different media after 7 days transformation was compared with CK as directly transformation. The effects of different 2,4-D concentration of differentiation pretreatments on callus regeneration frequency and regeneration process were investigated by using one inbred line and three hybrids immature embryos of maize as experimental materials. The results indicated that there was a significant increase on regeneration frequency with different 2,4-D concentration of pretreatments before transferred to differentiation medium.The pretreatment medium without 2,4-D could significantly improve the maize regeneration seedling frequency and accelerate the plant regeneration process. There was a significant different regeneration frequency with various genotype calluses. With the increase of 2,4-D concentration,the regeneration frequency of the same genotype callus was in a downward tendency in the several experimental genotypes. There was a highest regeneration rate on hybrids 87-1×Zheng 22 and no significant difference with other 2 hybrids. The plant regeneration frequency of three hybrids was generally higher than that of inbred line of 87-1. This demonstrated that the pretreatment with maize callus can increase the plant regeneration frequency and accelerate the regenerate process.
【Fund】: 河南省高等学校重点科研项目(15A180039);; 河南农业大学本科教学实验室开放项目(KF1613)
【CateGory Index】: S513
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 WU Hong1,XIE Shu-zhang1,LIN Qing1,LEI Kai-rong1,QIU Zheng-gao2,ZHANG Ya-qin2,WANG Nan2, ZHOU You-kun3 (1.Biotechnology Research Center,Key Laboratory of Adversity Agriculture,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;2.Institute of Maize,Chongqing Academy of Agricultural Sciences, Chongqing 401329,China;3.Beijing No.2 Middle School,Beijing 100009,China);Study on Callus Induction and Plantlet Regeneration from Immature Embyro among Different Maize Inbreds[J];西南农业学报;2012-02
2 ZHU You-yin1,2,ZHAO De-gang1,2,FENG Yi1,2,ZHAO Feng-lan1,ZHONG De-yi1,LIU Qian1(1.Guizhou Key Laboratory of Ago-Bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China;2.Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China);Study on Callus Induction and Plantlet Regeneration in Different Genotypes Maize[J];生物技术;2008-04
3 YANG Chun (Crops Research Institute in Cold & High Latitudinal Region,Shanxi Academy of Agricultural Sciences,Datong 037008,China);The Function of Growth Hormone and Cell Division Hormone in Potato Healing Tissue Differentiation[J];山西农业科学;2008-07
4 ZHANG Li-jun,ZHAO Cheng-hao,GE Chao(College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China);Regeneration System and Influencing Factors of Maize[J];玉米科学;2008-02
5 DU Wen-ping, XU Li-yuan, YU Gui-rong, WANG Yi, ZHONG Chang-song (Institute of Biotechnology & Nuclear Techniques, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China);System Establishment of Maize Immature Embryos Culture in Vitro[J];玉米科学;2007-02
6 JIANG Hua~(1,2,),CHEN Jing~(1,),GAO Xiao-Ling~1,WAN Jia~1,WANG Ping-Rong~1,WANG Xu-Dong~(1,3,*) and XU Zheng-Jun~(1,3,*)(()~(1,) Rice Research Institute,Sichuan Agricultural University,Wenjiang 611130,Sichuan;()~(2,)Department of Food and Bioengineering,Shandong Institute of Light Industry,Jinan 250100,Shandong;()~(3,)Key Laboratory of Crop Genetic Resources and Improvement Ministry of Education,Sichuan Agricultural University,Ya'an 625014,Sichuan,China);Effect of ABA on Rice Callus and Development of Somatic Embryo and Plant Regeneration[J];作物学报;2006-09
7 LI Yu-jing,CHEN Yan-long,WANG Ling-ling,ZHAO Song-min,ZHU Zheng-ge(College of Life Science,Hebei Normal University,Hebei Shijiazhuang050016,China);The Influence of 2,4-D and 6-BA on CultureAbility of Rice Callus[J];河北师范大学学报;2005-04
8 DENG Shi-zheng, FU Jin-feng, JI Liang-yue (Henan Agricultural University,Zhengzhou 450002,China);Study on Methods of Accelerating Plant Regeneration from Calli in Maize[J];河南农业大学学报;2005-01
9 XIAO Li jie,CANG Jing,XU Zhong,HOU Yan hua (Life Scientific College,Northeast Agricultural University,Harbin,Heilongjiang,150030,PRC);Callus induction and regeneration of inbred lines used largely in Heilongjiang[J];东北农业大学学报;2003-01
10 DU Juan, MU Qiu_hua, JIA Yu_feng, TIAN Li_guo, ZHANG Xin_sheng (Chang Chun University of Agricultural and Animal sciences,Chang Chun 130062 China);Studies on Increasing the Rate of Anther Culture Induction of Maize by the Method of Transform Breeding[J];玉米科学;1999-03
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 LI Hai-xia;SUN Jin-hua;XING Guo-zhen;WANG Ai-wu;He'nan Agricultural University;;Study on Improvement of Callus Regeneration Frequency in Maize[J];河北农业科学;2017-02
2 ZHAO Shi-quan;ZHA He-xia;ZHAO Hao;Nanjing Municipal Center for Disease Control and Prevention;;IC Determination of 2,4-Dichlorophenoxyacetic Acid in Fruits and Vegetables[J];理化检验(化学分册);2016-12
3 ZHU Keming;TAO Huimin;XU Shuo;DUAN Liqin;YANG Yanhua;;Research Progress of Rice Callus Differentiation and Regeneration[J];种子;2016-11
4 FENG Mingfang;CANG Jing;LU Qiuwei;ZHENG Chengcheng;ZHAO Jinmeng;LI Jiahong;QIN Lunan;YANG Wei;MENG Jing;ZHU Xiangchun;College of Life Scicece,Northeast Agricultural University;;Study on Factors of Establishment for Heilongjiang Japonica Rice Regeneration System[J];核农学报;2016-10
5 Yiliang QIAN;Yao GUO;Yanchao DING;Lifeng WANG;Jin GUO;Jun WANG;Yaocheng QI;Wei ZHANG;Xiaolong ZUO;Long RUAN;Tobacco Research Institute of Anhui Academy of Agricultural Sciences;Anhui Huaan Seed Co.Ltd.;Anhui Suixi Agricultural Science Research Experimental Station;Cereal Crop Research Institute,Henan Academy of Agricultural Sciences;;Methods for Producing Maize(Zea mays L.) Haploids and Their Application in Maize Breeding[J];Agricultural Science & Technology;2016-07
6 Feng Lu;Wang Yuguo;Wen Yinyuan;Gao Jiajia;Liu Yuan;He Meilin;College of Agriculture,Shanxi Agricultural University;;Effects of exogenous hormones on rapid propagation of purple potato plantlets in vitro[J];山西农业大学学报(自然科学版);2016-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Ru-tao,WANG Han-ning,KONG Wei-ping,WEN Rui-ting,JIA Xiao-xia,MA Huai-yi(College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China);Establishment of plant regeneration system from immature embryos of maize (Zea mays L.) skeleton inbred lines[J];甘肃农业大学学报;2009-06
2 WANG Shi-Yu1,ZHENG Yong-Lian 1,LIU Ya2,ZHAO Jiu-Ran2,and ZHANG Fang-Dong 1,(1 National Key Laboratory for Genetic Improvement of Crops,Huazhong Agricultural University,Wuhan 430070,Hubei;2 Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China);Transformation,Inducing and High-Frequency Regeneration of Embryogenic Callus Initiated from Mature Embryos of Maize(Zea mays L.)[J];作物学报;2008-03
3 GUO Xin-mei, ZHANG Xiao-dong, HAN Li-xin,CHEN Yao-feng (College of Animal Science and Technology,College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100 China; Beijing Agro-biotechnology Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China);Characteristic of callus induced from immature embryo among different maize inbreds[J];西北农林科技大学学报(自然科学版);2007-04
4 DU Wen-ping, XU Li-yuan, YU Gui-rong, WANG Yi, ZHONG Chang-song (Institute of Biotechnology & Nuclear Techniques, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China);System Establishment of Maize Immature Embryos Culture in Vitro[J];玉米科学;2007-02
5 XU Li-hua1,ZHANG Ju-ren2,ZHOU Zhu-hua1,XU Fang-zuo1,YIN Wei-jun1,XING Yan-ju1(1.Institute for Application of Atomic Energy,Shandong Academy of Agricultural Sciences,Jinan 250100;2.College of Life Science,Shandong University,Jinan 250100,China);Study on the Regenerative System of Maize Calli[J];玉米科学;2006-06
6 WANG Han-ning1,ZHANG Jin-wen1,KONG Wei-ping2,FENG Yu-lan1(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China; 2.Funong High-tech Seeds Limited Company of Gansu,Lanzhou 730070,China);Callus Initiation and Regeneration from Immture Embryos of Maize[J];玉米科学;2006-05
7 ZHAO Yun-yun~1,ZHOU Xiao-mei~2,WANG Guo-ying~3(1.Department of Biology,Capital Normal University,Beijing 100037,China;2.School of Life Science and Technology,Shanxi University,Taiyuan 030006,China;3.State Key Laboratory for Agrobiotechnology,China Agriculture University,Beijing 100094,China);Study on Tissue Culture and Transformation of Maize[J];山西大学学报(自然科学版);2006-03
8 ZHANG Ying,SUN Qing-quan,LIU Yu-zhen,CAO Mo-ju(The Maize Research Institue of Sichuan Agricultural University,Ya'an 625014,China);Research on the Production of Maize Embryogenic Callus by Immature Embryo as Transgenic Receptor[J];玉米科学;2006-04
9 LIN Gan et al(Department of Chemistry and Bioscience,Xiangfan University,Xiangfan,Hubei 441053);Study on the Tissue-culture of Growing Lamina from Corn Plant[J];安徽农业科学;2006-10
10 FU Feng-ling FENG Zhi-lei QU Bai-yan LI Wan-chen(Key Laboratory of Crop Genetic Resources and Improvement/Ministry of Education,Maize Research Institute,Sichaun Agricultural University,Ya'an,Sichaun 625014);RELATIONSHIPS BETWEEN INDUCTION RATE OF EMBRYONIC CALLUS AND ENDOGENOUS HORMONES CONTENT IN MAIZE[J];核农学报;2006-01
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