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Tissue structure analysis of patinopecten yessoensis

PANG Bao-cheng;WANG Xian-dai;LIANG Jiu-zheng;Aquatic Bureau of Luannan County;Agricultural University of Hebei;  
The experimental materials of patinopecten yessoensis were the experimental materials,master the method of making blood smear for patinopecten yessoensis,the tissue structures of patinopecten yessoensis were observed and analyzed by hematoxylin-eosin staining and tissue section.With 5mL syringe absorbs the heparin sodium 2mL,then extraction of grass carp 1mL of blood,on one end of the slide drip a drop of blood,the lift hand flat,right hand with another level of slide force will promote blood,air dry in the shade,make five blood smear in the same way.After drying,take one blood smear used as blank control,take two blood smear dyeing with Switzerland,another two blood smear with Jim dyeing liquid dye,air dry in the shade,wash,observed.Take the fresh tissue of patinopecten yessoensis tissue was cut into 1.0*1.0*0.3cm of the Scallop in Shell,after immobilization,dehydration and transparent,leaching wax,embedding process,the tissue block cut into 4~6μm paraffin sections.Then after hematoxylin staining hydrochloric acid alcohol to fade,with eosin stain re dyeing process with neutral gum complete mounting and dried for microscope observation and photography.Finally,the structure of the human body and fish was analyzed and compared with the observation and comparison.
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