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Study on extraction method of polysaccharide from radix isatidis

Zhang Hongjie,Wang Guangle(School of Biology and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China)  
The extraction method of radix isatidis polysaccharide is studied by using water extraction.Based on the single-factor experiment,a quadratic polynomial regression equation of the forecasting model is set up with Box-Behnken design of three factors and three levels.The optimal conditions of extraction are concluded as follows: extraction temperature of 90 ℃,extraction time of 6 h and liquid-solid ratio of 24 ∶ 1(mL·g-1).The extraction rate is 11.92% under these conditions.
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