Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Abcg 4expression in mouse tissues and its specific inhibition in the brain by RNA interference

Wang Xuefang,Li Xihai,Wu Jinmei(School of Biology and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China)  
Abcg4 was found highly expressed in the brain,eye and spleen by means of semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR),with higher expression in the first two organs.To further study the function of the gene,the effect of specifically designed small interference RNA(siRNA) on inhibiting this gene′s expression was investigated in vivo.These siRNAs were synthesized and injected into the mouse lateral ventricle with different dosage.Brains were immediately removed at different time point after injection,and Abcg4 mRNA expression was measured by RT-PCR.The result shows that 6ug of the specific siRNA can inhibit the expression of Abcg4 significantly,with an inhibition rate up to 85% 48 hours after injection.The study concludes that the expression of Abcg4 can be inhibited by its specific siRNA in the mouse brain.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved