Response of protective enzymes in Eupatorium adenophorum to simulation of insect bites and mechanical damage
ZHU Xunzhi;GAO Ting;LIU Sha;TANG Kai;YANG Congwei;School of Biotechnology,Jiangsu University of Science and Technology;Key Laboratory of Plant Biotechnology in Universities of Shandong Province,College of Life Sciences,Qingdao Agricultural University;
With the most destructive exotic invasive species Eupatorium adenophorum,we studied the activity of protective enzymes(T-SOD and POD) and the contents of total flavonoids and phenolic acid compounds in its roots and leaves responding to leaves holing as insects bites and plants cutting as mechanical hurts. The results showed that the treatments of leaves holing and plants cutting increased the activity of protective enzymes T-SOD and POD in leaves and roots significantly. The activity of T-SOD and POD in roots decreased and which in leaves increased along with the increasing number of holing and increasing extend of plants cutting. At the same time,the contents of total flavones and the phenolic acid compounds in roots and leaves reduced by the leaves holing and plant cutting. In the different treatments,with the increase of leaves holing and plant cutting,the contents of total flavones in roots and leaves increased,and phenolic acid in leaves increased but those in roots decreased. The result revealed that leaves holing as a simulation of insects bites and plants cutting as a simulation of mechanical damage,as two environmental stresses,have impacts on the antioxidant system of Eupatorium adenophorum leaves and roots. This study has a certain implication on improving the control efficiency and reducing the loss of agriculture and forestry.