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《Journal of Hubei University(Natural Science)》 2018-03
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The growth characteristics of the Polyodon spathula spleen cell based on the electric cell-substrate impedance sensing technology

LIU Juanjuan;TAN Chun;DU Hejun;WANG Binzhong;Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes,Institute of Chinese Sturgeon Research,China Three Gorges Corporation;  
Counting cells with a blood cell count plate is a common method of detecting cell proliferation,plotting cell growth curves,and studying cell growth characteristics. Electric cell-substrate impedance sensing( ECIS) technology is a highly innovative electrical methods,which can quantifying cell dynamic behavior without mark and damage. To determine the agreement between the two methods,the growth curves had been compared which determined by the ECIS technology monitoring system and the traditional cell counting method respectively in cultured paddlefish( Polyodon spathula) spleen cell line PSS1. The results demonstrated that the growth curves obtained with the ECIS technology monitoring system and the traditional cell counting method were in close agreement. Compared with traditional counting assay,the ECIS technology is a completely automated analysis method,and the experimental data has good repeatability,which is conducive to statistical analysis. Research results suggested that ECIS detection technology can not only replace the traditional cell counting method to study the growth characteristics of cells,but also has a wide range of applications in basic research and pharmaceutical fields.
【Fund】: 中国长江三峡集团公司内部项目(20130906)资助
【CateGory Index】: Q952
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