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Identification of Sonchus asper L. Powdery Mildew

ZHANG Qingchen1,PEI Dongli1,FENG Yiyang1,LI Chengwei2 ,HONG Quanchun1(1.Key Laboratory of Plant-Microbe Interactions,College of Life Science, Shangqiu Normal University,Henan Shangqiu 476000,China; 2.Key Laboratory of Plant Genetics and Molecular Breeding,Department of Life Science, Zhoukou Normal University,Henan Zhoukou 466001,China)  
In order to make clear the phylogenetic evolution relationship of Sonchus asper L.powdery mildew detected in Shangqiu,China for the first time,morphological,etiological and molecular systematic identification were tested.Results show that conidiophores of pathogenic bacteria are cylindrical,vertical,simple and unbranched,108~220 μm×10~12 μm,with one foot cell and 2~3 short cells.Conidia are in cluster,cylindrical,no obvious fibrosin body,29~42 μm×19~24 μm.Mycelial appressorium shows a nipple-shaped.Pathogen in natural state has the same symptom to inoculated leaves with white patch having thin and unconspicuous border.Internal transcribed spacer(ITS) sequences of its ribosomal DNA were amplified by PCR and sequenced to obtain the 595 bp sequence(GenBank: JQ010848),and sequence analysis by MEGA3.1 software reveals that sequences of pathogenic bacteria and AY739111,HM449077,AB453762,AB077673 from Golovinomyces cichoracearum gather together,being distantly related to ITS sequences from other three species in the same genus.The research shows that pathogen of Sonchus asper L.powdery mildew in Shangqiu is G.cichoracearum.
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