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Removing Technology Optimization of Auto-growth Inhibitors of Spirulina platensis by Ultrafiltration Membrane

YU Shuimiao;WU Xia;WANG Jianming;CONG Wei;LU Yunming;College of Food Engineering and Biotechnology,Tianjin University of Science and Technology;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences;Shenzhen Lvdebao Health Food Limited Company;  
The method of ultrafiltration technique was adopted for separation of auto-growth inhibitors of Spirulina platensis.The effect of molecular weight cut offs(MWCOs)of membrane on removal efficiency was investigated.Ultrafiltration conditions for the auto-growth inhibitors of Spirulina platensis extracts from used culture medium were optimized by orthogonal array design based on membrane flux.It is indicated that 100 kDa can reduce autogrowth inhibitors effectively,and the optimized ultrafiltration conditions were found to be 0.20 MPa,450r/min,35 ℃ and 9for pressure,mixing speed,temperature and sample pH,respectively.Cleaning with deionized water at35 ℃for 0.5hwas the best method for membrane cleaning,and the flux recovery rate of membrane after cleaning was about 94%.
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