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Influence and Mechanism of Aerobic and Exhausted Swimming Exercise on Immune Responses of DNA Vaccine

SONG Hongli;NIU Yingpeng;WANG Junpeng;Henan University Translational Medicine Center of Huaihe Clinical College;Henan University College of Physical Education;Henan University Translational Medicine Center of Huaihe Hospital;Henan University Institute of Molecular Medicine;  
Forty-eight female C57BL/6mouse were randomly divided into three groups(16mouse/group):control,aerobic(AE),and exhausted(EE)swimming exercise groups.To evaluate the effects of aerobic and exhausted exercise on DNA vaccine,the humoral and cellular immune responses were determined in the swimming mice immunized with DNA vaccine for three times.In addition,to elucidate how AE/EE enhance/impair the immune response in a swimming mouse model,the frequency of CD4~+CD25~+regulatory T cells(Treg)and the production of IL-2from spleen cells were determined by flow cytometry assay after the completion of six weeks of swimming exercise of mice.Here our data show that,compared with control group,aerobic swimming exercise could augment cell mediated immunities(CMI)such as increasing IFN-γexpression,T cell proliferation and antigen-specific cytotoxic T lymphocyte(CTL)responses in the animal immunized with HBV DNA vaccine.In contrast,the CMI and CTL responses were impaired by exhausted swimming exercises.In addition,both aerobic and exhaustedswimming exercise did not affect antigen-specific IgG levels from serum.Further mechanism study showed that exhausted swimming exercise increased the frequency and inhibitory function of Treg cells;whereas,aerobic exercise did not affect Treg cell frequency and function but increase the IL-2production.These data indicate that 1)both aerobic and exhausted swimming exercise can not affect the humoral response,2)aerobic exercise has the ability to enhance the cellular immune responses of DNA vaccines possibly via increasing the IL-2production of splenocytes,and 3)exhausted exercise can inhibit the cellular immune responses of DNA vaccines possibly via enhancing the number and inhibitory function of Treg cells.
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