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《Journal of Harbin Institute of Technology》 2011-10
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Composition optimization of medium for a syntrophic acetogen coculture

LI Jian-zheng1,GAO Chen-chen1,2,ZHANG Li-guo1,LIU Chong1,JIN Yu1,ZHANG Yan1(1.State Key Laboratory of Urban Water Resource and Environment,School of Municipal and Environmental Engineering, Harbin Institute of Technology,150090 Harbin,China;2.National Engineering Research Center for Urban Water & Wastewater,300074 Tianjin,China)  
Hydrogen-producing acetogens,between acidogenes and methanogens in trophic niche,is very difficult to be separated and only few of the pure cultures have been obtained.This has restricted the development of new anaerobic organic wastewater treatment process with high efficiency based on enhancing their metabolic capability.A new syntrophic acetogen coculture named 7-m-2a which showed great capacity in degrading propionic acid was introduced in this paper.To optimize the culture medium,the influences on 7-m-2a's growth and metabolism of propionic acid concentration,nitrogen sources,Fe2+,Mg2+,Ca2+ and pantothenic acid were investigated.The results indicated that the suitable concentration of propionic acid and Fe2+,Mg2+,pantothenic acid were 10 g/L and 88,38,30 mg/L,respectively.The optimum nitrogen source was the mixture of yeast extract,tryptone and NH4Cl with a concentration of 0.33 g/L respectively.With the optimization culture,a specific degradation rate of propionic acid and acetic acid yield of 998 mg/(L·d) and 3 947 mg/L,respectively,obtained after fermenting for 30 days at 38 ℃.
【Fund】: 国家自然科学基金面上项目(51178136);; 哈尔滨市科技创新人才专项基金项目(2009RFXXS004)
【CateGory Index】: TQ920.1
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