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《Chemical Industry Times》 2012-06
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Determination of Syringin in Acanthopanax Senticosus by High Performance Capillary Electrophoresis

Dou Guodong(Chemistry and Chemical Engineering,Guizhou University,Guizhou Guiyang 550025)  
To develop method for determination of determination of syringin in Acanthopanax senticosus.syringin were determined by high performance capillary electrophoresis(HPCE) under the optimized condition: the running buffe of 15 mmol·L-1 Sodium dihydrogen phosphate-20 mmol·L-1 sodium borate-20%ethanol(pH8.5),voltage 25 kV,λ=294 nm.,and capillary temperature 25℃.The linearity of the calibration curves for syringin was established in the ranges of 0.0129 ~0.258 mg·mL-1(r=0.999 8),The average recovery was 99.78%,RSD1.09%.The method is simple,rapid and reproducible,and it can be used for the quality control of Acanthopanax senticosus.
【CateGory Index】: R284
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