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《Environmental Science》 2007-01
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Effects of Cadmium Exposure on the Mitochondrion Structure and Energy Metabolism in Gill of Tilapia Oreochromis niloticus

BAI Shi-jun,XU Zi-rong(Feed Science Institute of Zhejiang University,Hangzhou 310029,China)  
To explore the effects of waterborne cadmium(Cd) on the mitochondrion structure and cellular energy metabolism in gills of tilapia(Oreochromis niloticus) and possible mechanisms,specimens of tilapias were exposed to different Cd~(2+) concentrations(0,50,500(μg/L) freshwater) for 7 days under indoor simulate condition.At the end of the experiment,ultrastructure and superoxide dismutase(SOD) activity as well as malondialdehyde(MDA) level of branchial mitochondria were detected,while the phosphofructokinase(PFK) and ATPase activities and the contents of lactic acid(LD),ATP,ADP,AMP in gill tissues and osmolality,Na~+,K~+ and Ca~(2+) concentrations in plasma were assayed,and the energy charge(EC) was also calculated.The results showed that the structure of mitochondria in fish exposed to 50(μg/L) Cd~(2+) was intact and there were no significant differences except the significant elevation in SOD activity in all biochemical indicators examined as compared to the control(p0.05).However,in fish exposed to 500(μg/L) Cd~(2+),mitochondria were damaged severely,SOD and ATPase activities were decreased significantly whereas the contents of LD,MDA,ADP and AMP as well as concentration of K~+ in plasma in comparison with the control values were enhanced significantly(p0.05),in addition,there was a decreasing trend in PFK activity and plasma osmolality though the difference was not significant(p0.05).The present findings suggested that the short-term exposure to higher concentrations of Cd~(2+) would lead to the obstruction of energy supply and utilization due to inhibited ATPase and PFK activity as well as oxidative damage to mitochondria resulting from decreased SOD activity,and decrease the ability of gills to regulate the plasma ion composition and osmolality,which would be one of the possible mechanisms for Cd~(2+) toxicity.
【Fund】: 国家自然科学基金项目(30471255);; 国家“985工程”创新平台项目
【CateGory Index】: X174
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