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《Environmental Science》 2009-04
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Microbial Community Constitute and Phylogenetic Analysis on Oil Polluted Soils in Oilfields of China

JIA Jian-li~1,LI Guang-he~2(1.School of Chemical & Environmental Engineering,China University of Mining & Technology(Beijing),Beijing 100083,China;(2.Department) of Environmental Science and Technology,Tsinghua University,Beijing 100084,China)  
The microbial communities in oil polluted soils from oilfields of China were analyzed using a modern molecular biological approach.The crude total DNA from soils were directly extracted,16S rDNA fraction were amplified by PCR,the bands were separated using denaturing gradient gel electrophoresis(DGGE),and some of the typical bands were sequenced and compared with the records of NCBI to classify the germs.Comparison of the DNA extraction indicated that the yields were 1.4-2.2 times as much as the existing technique,and the DNA purity reached 1.8-2.0.With higher similarity and correlation in CQ and DQ oilfields but lower in that of SL and YM oilfields,the influence factors to microbial communities consisted in the elementary properties of soil samples such as oil contents,water ratio,etc.Extraction and sequencing analysis of selected 16S rDNA bands demonstrated a range of similarity of 89%-100% to reference bacteria.The Shannon-Weiner index of the oil contaminated soils was 0.5-1.2,and which was increased slightly along with the enhancement of microbial amounts and FDA activities.According to this research,it has possibility in developing a reliable tool for researching oil contaminated soil bacteria diversity,and in applying a credible foundation for adjusting the microbial ecosystem and determining the dominant community in contaminated soils in oilfields.
【Fund】: 科技部平台项目(2004DEA70890)
【CateGory Index】: X172
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Chinese Journal Full-text Database 1 Hits
1 HUANG Yong-hong1,2,YUAN Hong-mei2,WU Xiao-lin3,SONG Kao-ping1(1.Petroleum Engineering College,Daqing Petroleum Institute,Daqing,Heilongjiang 163318,China;2.Life Science Department,Daqing Normal University,Daqing,Heilongjiang 163712,China;3.Exploration and Development Institute,Daqing Oilfield Co.Ltd.,Daqing,Heilongjiang 163712,China);Analysis of microbial community structure for the Daqing oil field in low-permeability reservoir block areas[J];Journal of Daqing Petroleum Institute;2009-04
Chinese Journal Full-text Database 10 Hits
1 Guanghe Li1,Xu Zhang1,Wei Huang2(1Dept.of Environmental Science and Engineering.Tsinghua University,Beijing 100084,China Email:ligh@mail.tsinghua.edu.cn;2Dept.of Civil and Structural Engineering,University of Sheffield,Mappin Street,Sheffield Sl 3JD,UK);Characteristics of Degrading Microorganism Distribution in Polluted Soil with Petroleum Hydrocarbons[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;2000-04
2 JIA Jian-li, LI Guang-he, ZHONG Yi (Department of Environmental Science and Technology, Tsinghua University, Beijing 100084, China;The Relationship Between Abiotic Factors and Microbial Activities of Microbial Eco-system in Contaminated Soil with Petroleum Hydrocarbons[J];Environmental Science;2004-03
3 WU Yong-sheng,LI Hao,MA Wan-li,LU Ping,L Gui-fen(Biodiversity Conservation Center,College of Life Sciences and Technology,Inner Mongolia Normal University,Huhhot 010022,China);A Review of Molecular Biology Techniques for Studying Soil Microorganisms in Grassland[J];Journal of Inner Mongolia Normal University(Natural Science Edition);2008-02
4 XU Xiao-Yu 1 MIN Hang1 ** LIU He 2 WANG Yuan-Peng 2 (1. College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 2. College of Environment Engineering, Zhejiang University, Hangzhou 310029, China);Comparison of DNA Extraction Methods for PCR-DGGE Analysis of Bacterial Community in Soil[J];Journal of Agricultural Biotechnology;2005-03
5 MA Yue-Xin{1,2}, Carola Holmstrm2, Jeremy Webb2, Staffan Kjelleberg2 (1.School of Life Science and Technology, Dalian Fishery University 116023,China;2.School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia).;Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology[J];Acta Ecologica Sinica;2003-08
6 Teng Ying Luo Yongming  Zhao Xiangwei Li Zhengao Song Jing Wu Longhua (State Key Laboratory of Soil and Agricultural Sustainable Development (Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China) (Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China);RAPID EXTRACTION AND PURIFICATION OF DNA IN FARMLAND SOILS CONTAMINATED WITH MIXED HEAVY METALS FOR PCR-DGGE ANALYSIS[J];Acta Pedologica Sinica;2004-03
7 JIANG Xin 1,2 MA Ming-Chao1 LI Jun 2 LI Li2 ZHONG Zuo-Shen1(School of Water Resources and Environment,China University of Geosciences/Beijing Key Laboratory of Water Resources & Environmental Engineering,Beijing 100083)1(Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agriculture Science,Beijing 100081)2;Analysis of Microbial Community Distribution in Constructed Rapid Infiltrition System (CRI) by DGGE[J];Microbiology;2007-06
8 Chen Hao Tang Xiaoshu\ Lin Jie\ Zhang Bosheng\ Ren Daming (The State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China);Community Constitute and phylogenetic Analysis on Soil Uncultured Microorganism[J];Acta Microbiologica Sinica;2002-04
9 Zhang Ruifu 1 Cao Hui 1,2 Cui Zhongli 1** Li Shunpeng 1 Fan Ben 1 ( 1Key Lab of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University,Nanjing 210095,China) ( 2Institute of Soil Science, the Chinese Academy of Sciences, Nanjing 210008, China);Extraction and Purification of Soil Microbial Total DNA[J];Acta Microbiologica Sinica;2003-02
10 HUANG Yao rong,LI Deng yu,ZHANG Xiao ping,LIANG Ru yu,CHENG Qiang (Sichuan Agriculture University,Ya’an 625014,China);Studies on microbial activity and dominant microorganisms in soil polluted by pentachorphenante-Na(PCP -Na)[J];SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES;1999-S1
Chinese Journal Full-text Database 4 Hits
1 SHI Mei,et al.(Exploration and Development Research Institute of Daqing Oilfield Company Ltd., Daqing 163712,China);Organism distribution of source microbe in Daqing oil reservoirs[J];Petroleum Geology & Oilfield Development in Daqing;2004-05
2 BAO Mu-tai~(1,2), KONG Xiang-ping~1, SONG Yong-ting~2, CHEN Yong~2, WEI Bin~2, ZHANG Song-ting~2, WANG Wei-dong~(1,2), WANG Xiu-lin~1(1.College of Chemistry & Chemical Engineering in Ocean University of China, Qingdao 266003, China; 2.Research Institute of Oil Production Technology in Shengli Oilfield Company,China Petrochemical Corporation, Dongying 257000, China);Selective activation condition of stratal microflora of S12 block in Shengli Oilfield[J];Journal of the University of Petroleum,China;2004-06
3 Huang Xue~1 Wu Xiaolin~2 Hou Zhaowei~2 (1.College of Geosciences and Resources,China University of Geosciences,Beijing 100083,China;2.Exploration and Development Research Institute,Daqing Oilfield Company,Ltd.,Daqing 163712,China);Mechanismof degradation for petroleumhydrocarbon by Brevibacillus brevis and Bacillus cereus[J];Acta Petrolei Sinica;2006-05
4 Yang Zhenyu Shi mei Wang Dawei Chang Jianfei Dou Xumou ( Research Institute of Exploration and Development, PetroChina Daqing Oilfield Company Ltd. ,Daqing 163712,China; Daqing Petroleum Institute, Daqing 163318,China);Study on authigenous microorganism community distribution and oil recovery mechanism in Daqing Oilfield[J];Acta Petrolei Sinica;2006-S1
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jin-bing~(1,2), ZHANG Yong-hua~2, YANG Xiao-mao~3, FU Li~3, ZHONG Zuo-shen~4 (1.Beijing University of Science & Technology, Beijing 100083,China; 2.North China Institute of Water Conservancy and Hydroelectric Power, Zhengzhou 450011,China; 3.Bejing University Shenzhen Graduate School, Shenzhen 518057,China; 4.China University of Geosciences, Beijing 100083,China;);On-site Experimental Study on the Treatment of Polluted River Water by Constructed Rapid Infiltration System[J];Journal of North China Institute of Water Conservancy and Hydroelectric Power;2004-03
2 YE Yang-fang1,2, MIN Hang2,*, Liu Zhen-mei2 (1. College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China. 2. College of Life Science, Zhejiang Un iversity, Hangzhou 310029, China);Monitoring impact of mefenacet treatment on soil microbial communities by PCR-DGGE fingerprinting and conventional testing procedures[J];环境科学学报(英文版);2006-02
3 Xu Zhang 1,Guanghe Li 1,Wei Huang 2(1 Dept of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China E\|mail:zhangxu@mail tsinghua edu cn; 2 Dept of Civil and Structural Engineering,University of Sheffield,Mappin Stree;Effect of Temperature on Biodegradation of the Petrochemicals in Contaminated Soil[J];Chinese Journal of Enviromental Science;2001-04
4 XING De-feng, REN Nan-qi, GONG Man-li (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Application of PCR-DGGE to Resolve Microbial Diversity in Bio-Hydrogen Producing Reactor[J];Environmental Science;2005-02
5 ZHAO Yang-guo, REN Nan-qi, WANG Ai-jie, LIU Guang-min, ZHAO Qiu-shi, SHANG Huai-xiang (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Microbial Community Structure Analyzed by Single-Strand Conformation Polymorphism Technique in Sulfate-Reducing Reactor[J];Environmental Science;2005-04
6 XING De-feng~1,REN Nan-qi~1,SONG Jia-xiu~1,QU Min~(2,3),XU Xiang-ling~2(1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.College of Life and Environmental Science,Harbin Normal University,Harbin 150080,China;3.College of Food Engineering,Harbin University of Commerce,Harbin 150076,China);Community of Activated Sludge Based on Different Targeted Sequence of 16S rDNA by Denaturing Gradient Gel Electrophoresis[J];Environmental Science;2006-07
7 ZHAO Yang-guo,WANG Ai-jie,REN Nan-qi,ZHAO Yan(School of Environmental and Municipal Engineering,Harbin Institute of Technology,Harbin 150090,China);Microbial Community Structure in Different Wastewater Treatment Processes Characterized by Single-Strand-Conformation Polymorphism(SSCP) Technique[J];Environmental Science;2006-07
8 Ahang Aiyun Cai Daoji (Nanjing Institute of Environmental Science, NEPA);EFFECTS OF HERBICIDE ON THE MICROBIAL ACTIVITY, NITRIFICATION AND AMMONIFICATION IN SOILS[J];Rural Eco-environment;1990-03
9 WANG Juan1,GU Xue-jing2,ZHAO Ji3(1.Faculty of Natural Resource and Information Technology,CUP,Beijing 102249,China;2.Research Institute of Forest Ecology,Environment and Protection,CAF,Beijing 100091,China;3.College of Life Science,Inner Mongolia University,Hohhot 010021,China);Function of Soil Enzyme Activities in Indicating Soil Fertility in Leyluse Chinensis Steppe[J];Journal of Agro-Environment Science;2006-04
10 LU Gui fen 1,2 (1. Department of Ecology & Environmental Science, NeiMongol University, Hohhot 010021, PRC; 2. Biology Department, NeiMongol College of Education, Hohhot 010010, PRC);Study on Seasonal Changes for the Number of Microorganism in the Process of Litter Decomposition of Artemisia Ordosica Communities[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL;2000-01
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