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《Journal of Navy Medicine》 2001-04
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The Effect of Cellullar and Humoral Immunosuppression on Nitric Oxide and Nitric Oxide Synthase and Its Isoenzyme in the Acute Rejection of Golden Hamsters to Rat Liver Xenografts

DIAO Tong jin, YUAN Tong ye, LI You lin, et al (The General Surgery and Hepatobiliary Surgery Department, No. 401 Hospital of PLA, Qingdao 266071, China)  
Objective: To evaluate the effect of cellullar and humoral immunosuppression on nitric oxide, nitric oxide synthase and its isoenzyme in acute rejection of golden hamsters to rat liver xenografts. Methods: There were five randomly divided groups (n=6~9): isografts (group Ⅰ), xenografts in acute rejection (group Ⅱ), xenografts using cyclosporine (group Ⅲ), xenografts using cyclophosphamide in combination with splenectomy (group Ⅳ), and xenografts using splenectomy, cyclophosphamide and cyclosporine (group Ⅴ). The levels of serum ALT, TNF α, and nitric oxide production (NOx) in recipients were examined. Expressions of type Ⅱ (iNOS) and type Ⅲ (cNOS) nitric oxide synthase (NOS), inducible NOS (iNOS) and constitutive NOS (cNOS) were observed by NADPH diaphorase histochemical and immunohistochemical staining. Results: The levels of serum ALT activity of serum TNF α and systemic levels of NO metabolite in group Ⅱ and Ⅳ were higher than those of group Ⅰ and Ⅴ (P0.01). Cyclosporine in group Ⅲ can repress the cellular immune response, the synthesis of nitric oxide and the expression of NO synthase, but can not prolong the liver xenograft survival. The overexpression of NOS, iNOS and cNOS in liver xenograft rejection in group Ⅱ and Ⅳ was detected by NADPH diaphorase histochemical and immunohistechemical staining. Conclusion: Hepatocyte derived nitric oxide may be protective in the hyperesponsive state, but hepatic injury is likely to be triggered by the centrilobular iNOS expression in the superresponsive state.
【CateGory Index】: R657.3
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 DIAO Tong-jin; DENG Li-hua3; LI Dong-mei, et al (1. Department of General Surgery and Hepatobiliary surgery, Chinese PLA 401 Navy Hospital, Tsingdao 266071, China: 2. Eastern Hospital of Hepatobiliary Surgery, Chinese PLA Second Military Medical Universi;Functional roles of nitric oxide pathway during ischemia-reperfusion injury in the rat orthotopic liver transplamation[J];BASIC MEDICAL SCIENCES AND CLINICS;2000-01
2 DIAO Tong Jin 1, LI Wei Hua 1, WU Meng Chao 2, YAO Xiao Ping 2, YANG Jia Mei 1, LI Dong Mei 1, JI Bing 1 and LI Feng Chi 1 1Department of General Surgery and Hepatobiliary Surgery, Chinese PLA 401 Navy Hospital, Qingdao 266071, Shand;Cellular localization of nitric oxide synthase during acute rejection in golden harmster to rat orthotopic liver xenotransplantation[J];WORLD CHINESE JOURNAL OF DIGESTOLOGY;1999-10
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 MA Zheng-wei 1, LIU Li-dong 2, LI Kun 1, ZHANG Yu-jun 1, Chen Li-jun 1, DONG Jia-hong 1 ( 1Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038; 2Department of General Surgery, No.141 Hospital of PLA, Qingdao 266100, Shandong province, China);Establishment and assessment of TPN model in rats with liver transplantation[J];Acta Academiae Medicinae Militaris Tertiae;2004-22
2 DIAO Tongjin,LI Youlin,ZHAO Xiaodong,et al. The general surgery and hepatobiliary surgery department,the Chinese Navy 401th Hospital of PLA,Qingdao 266071;The function of nitric oxide in acute rejection of golden hamster to rat orthotopic liver xenotransplantation and studies of NADPH-diaphorase histochemistry[J];Journal of Hepatopancreatobiliary Surpery;2000-04
3 HE Ming-yan,CAO Nong,LI Yu-min,et al. Department of General Surgery,the First Affiliated Hospital of Lanzhou Medical College,Lanzhou 730000;An improved model of orthotopic liver transplantation in rats with three cuff technique for vascular anastomoses[J];Journal of Hepatopancreatobiliary Surgery;2003-04
4 DIAO Tong-jin; DENG Li-hua3; LI Dong-mei, et al (1. Department of General Surgery and Hepatobiliary surgery, Chinese PLA 401 Navy Hospital, Tsingdao 266071, China: 2. Eastern Hospital of Hepatobiliary Surgery, Chinese PLA Second Military Medical Universi;Functional roles of nitric oxide pathway during ischemia-reperfusion injury in the rat orthotopic liver transplamation[J];BASIC MEDICAL SCIENCES AND CLINICS;2000-01
5 Rui Li,Wei Zhong Wang,Jian Jun Yang,Gang Ji,Ji Peng Li,Yi Lei, Rui Li1,Wei Zhong Wang1,Jian Jun Yang1,Gang Ji1,Ji Peng Li1,Yi Lei2,1Department of General Surgery,2Department of Anesthesiology,Xijing Hospital,Fourth Military Medical University of Chinese PLA,Xi'an 710032,Shaanxi Province,China;Changes of serum nitric oxide and activities of nitric oxide synthase and inducible nitric oxide synthase after small bowel transplantation in rats[J];Chinese Journal of Clinical Rehabilitation;2004-20
6 JIN Xiu-hong,WANG Qun-si,SHEN Zhao-bo,et al.Zhengzhou Children's Hospital,Zhengzhou 450053,China;Study on the 5' regulation region deletion in iNOS gene of children with asthma[J];Chinese Journal of Practical Pediatrics;2008-04
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