Ischaemia-reperfusion of Lower Extremities Induces Cardiac and Pulmonary Injury in Rats
HU Jia le (Department of General Surgery, No. 411 Hospital of PLA, Shanghai 200081, China)
Objective:To evaluate the injury caused by lower extremity ischaemia reperfusion (IR) to the rat hearts and lungs.Methods:Animals were randomized into two groups. Group Ⅰ that underwent laparotomy and the abdominal aorta were clamped below the renal artery level for 120 minutes, and then were declamped for another 120 minutes. Group Ⅱ were operated without being clamped. Myocardium ATPase, myocardium apoptosis and mean artery pressure (MAP) were observed; so were pulmonary malonyldialdehyde (MDA), concentration of Evan's blue and glucocorticoid receptor (GR) mRNA level. Results:In group Ⅰ, myocardium and alveolar wall were infitrated by neutrophils and lymphocytes; myocardium degenerative changes and pulmonary edema could also be seen microscopically. Myocardium ATPase activities decreased, while apoptosis cells increased significantly. MAP was lower in group Ⅰ than in group Ⅱ. Pulmonary MDA, Evan's blue concentration increased in group Ⅰand the GR mRNA level decreased.Conclusion:Lower extemity IR may cause myocardium and pulmonary injury, which induces dysfunction of the two organs.
【CateGory Index】： R364.12