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《Journal of Navy Medicine》 2001-04
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Eukaryotic Expression and Bioactivity Determination of Recombinant Human Interleukin 12 Gene

LONG Jian qiu, GAO Jun, LI Mao, et al (Naval Medical Research Institute, Shanghai 200433, China)  
Objective: To assess the expression capability of the previously constructed eukaryotic expression vectors carrying the recombinant human interleukin 12(hIL 12) gene. Methods:The recombinant hIL 12 eukaryotic expression vectors were transfected by the 293 cells, and the supernatant was harvested 72 hours after the transfection. The enhanced effect in natural killer activity due to the addition of the post transfection cell supernatant was examined by LDH and was compared with the IL 12 standard sample. Results: The IL 12 eukaroytic expression vectors constructed previously in our laboratory can express the IL 12 protein and its concentration was between 2.5~5.0 ng/ml. Conclusion: This method can be applied to measuring the bioactivity of the IL 12 and thereby assessing the expression capability of the constructed vectors.
【CateGory Index】: R329-33
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