Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Navy Medicine》 2001-04
Add to Favorite Get Latest Update

Influence of LPS on the Levels of Gsα and Giα Protein Subunits in Pulmonary Microvascular Cell Membrane

ZHANG Jun feng, HUANG Xian bin, SUN Geng yun, et al (No. 442 Hospital of PLA, Ningde 352102, China)  
Objective:To observe the influence of lipopolysaccharide(LPS) and methylprednisolone sodium succinate on G protein subunits in rat pulmonary microvascular cell(RPMVECs) membrane.Methods:The level of G protein subunits in RPMVECs membrane was examined by flow cytometry(FCM). Results: ①30 min and 90 min after treatment with 10 μg/ml LPS, the levels of Gsα protein subunits decreased significantly compared with the control group. After methylprednisolone sodium succinate had been given, the effects of LPS on the level of Gsα protein subunits in RPMVECs membrane were inhibited. ②30 min and 90 min after treatment with 10 μg/ml LPS, the level of Giα protein subunits markedly increased compared with the control groups. After methylprednisolone sodium succinate had been given, the effects of LPS on the level of Giα protein subunits in RPMVECs membrane were inhibited. Conclusion: ①Alteration of the levels of Gsα and Giα protein subunits induced by LPS may be an important mechanism in the increase of permeability of RPMVECs induced by LPS. ②Methylprednisolone sodium succinate was involved in inhibiting the increase of permeability of RPMVECs monolayer induced by LPS.
【Fund】: 国家自然科学基金项目 ( 396 0 0 0 6 2 )
【CateGory Index】: R363
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Xiao Zhenliang, Sun Gengyun, Huang Biqiong et al. Xinqiao Hospital, The Third Military Medical University, Chongqing 400037;EFFECT OF INFLAMMATORY INJURY ON BETA ADRENOCEPTOR IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL CELLS[J];解放军医学杂志;2000-02
2 Yang Qin; (Department of Pathophysiology, Guiyang Medical College, Guiyang 550004);The changes of G protein in anterior pituitary cell membranes from old rats[J];中华老年医学杂志;1996-02
3 Sun Gengyun,Mao Baoling,Lu Baozhang(The 2nd Affiliated Hospital,Third Military Medical College,Chongqing 630037);CHANGE 0F PULMONARY BETA─ADRENERGIC RECEPTOR AND ITS RELATI0N TO METABOLISM 0F MEMBRANOUS PHOSPHOLIPIDS DURING ENDOTOXIN─INDUCED RAT LUNG INJURY[J];中国应用生理学杂志;1994-01
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 LI Hui,SUN Geng-yun,FEI Li-ming,SHEN Ji-long.Department of Respiratary Medicine.The First Affiliated Hospital of Anhui Medical University.Hefei 230022,Anhui,China;Studies on the expression of Src-suppressed C kinase substrate mRNA in pulmonary mirovascular endothelial cells induced by lipopolysaccharide[J];中国危重病急救医学;2008-02
2 Xiao Zhenliang1, Yang Dachun1, Zhang Yuhua1,Sun Gengyun2,Qian Guisheng3.1.Intensive Care Unit,Chengdu Army General Hospital,Chengdu 610083;2.Department of Respiratory Medicine,the First Affiliated Hospital,Anhui Medical University,Hefei 230022;3.Department of Respiratory Medicine,the Second Affiliated Hospital,the Third Militory Medical University,Chongqing,400037,China;Effects of TNFα on cAMP in Rat Pulmonary Microvascular Endothelial Cells[J];医学研究杂志;2007-06
3 ZHAO Qing, HUANG Hai-xiao, JIN Chun-hua (The First Military Medical University, Guangzhou 510515, China);The regulation and its mechanism of Polydatin on the β-adrenoreceptor in cardiac myocytes stimulated by lipopolysaccharide[J];中国药理学通报;2004-07
4 XIAO Zhen liang, QIAN Gui sheng, SUN Geng yun (Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of tumor necrosis factor alpha on beta-adrenoceptor and beta-adrenoceptor related GRKs in rat PMVEC[J];第三军医大学学报;2004-10
5 Xiao Zhenliang, Qian Guisheng, Sun Gengyun et al . Insititute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;EFFECTS OF LIPOPOLYSACCHARIDE ON β-ADRENOCEPTOR AND β-ADRENOCEPTOR KINASES IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL CELLS[J];解放军医学杂志;2003-02
6 ZHANG Jun feng, HUANG Xian bin, SUN Geng yun, et al (No. 442 Hospital of PLA, Ningde 352102, China);Influence of LPS on the Levels of Gsα and Giα Protein Subunits in Pulmonary Microvascular Cell Membrane[J];海军医学杂志;2001-04
7 Xiao Xueyuan 1) Ge Xueming 2) An Mingbang 2) Lü Baozhang 2) ( 1)Education Ministry Key Laboratory of Cell Proliferation and Regulation Biology, Beijing Normal University, 100875, Beijing, China; 2)Institute of Basic Medical Scie;RELATIONSHIP BETWEEN PHOSPHOLIPASE A_2 ACTIVATION MEDIATED BY G PROTEIN βγ SUBUNIT AND β-ADRENERGIC RECEPTOR DESENSITIZATION[J];北京师范大学学报(自然科学版);2000-06
8 Xiao Zhenliang, Sun Gengyun, Huang Biqiong et al. Xinqiao Hospital, The Third Military Medical University, Chongqing 400037;EFFECT OF INFLAMMATORY INJURY ON BETA ADRENOCEPTOR IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL CELLS[J];解放军医学杂志;2000-02
9 WANG Xiao-wei LIU Feng-qi.Department of Cardiology,the First Clinical Medical College,Harbin Medical University,Harbin 150001,China;Relation of nerve signal transduction function and chronic obstructive pulmonary disease[J];国际呼吸杂志;2008-16
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Sun Gengyun,Mao Baoling,Lu Baozhang(The 2nd Affiliated Hospital,Third Military Medical College,Chongqing 630037);CHANGE 0F PULMONARY BETA─ADRENERGIC RECEPTOR AND ITS RELATI0N TO METABOLISM 0F MEMBRANOUS PHOSPHOLIPIDS DURING ENDOTOXIN─INDUCED RAT LUNG INJURY[J];中国应用生理学杂志;1994-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved