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《Journal of Heilongjiang Bayi Agricultural University》 2017-03
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Preparation of Cattle Pasteurella multocida Ghosts

Chang Chunlong;Wang Shixia;Zhai Junjun;Ni Hongbo;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;  
The temperature-sensitive lysis cassettes containing phage Phi X174 lytic gene E and start repressor system CI857-PLPR was amplified from plasmid p HH43 by PCR,the lysis cassettes CI857-PL-PR-E was inserted into shuttle vector p PBA1100,the recombinant plasmid p PBA1100-E was conjugated into cattle P. multocida by the experiment of conjugation,the lysis efficiency in P. multocida were detected by lysis dynamics test. The results showed that the lysis plasmid p PBA1100-E was successfully constructed,and it was transferred into P. multocida. The result of lysis dynamics test indicated that the lysis plasmid p PBA1100-E in Cattle P. multocida had a high lysising efficiency,the lysising efficiency was 99.98%. The cattle P. multocida ghosts was successfully prepared in this experiment,which laid the foundation for the further development of the cattle P.multocida ghost vaccine.
【Fund】: 黑龙江省博士后科研启动金资助(LBH-Q14134)
【CateGory Index】: S852.61
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 Zheng Jiasan;Liu Jiannan;Wu Ling;Zhang Hongyou;Xu Chuang;Xia Cheng;Lv Yanqing;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;Animal Husbandry and Fisheries Service Center of Bayan Chagan Town,Durbat Monggol Nationality Autonomous County;;Research on Deficiency of Main Vitamins and Trace Elements in Lactating Dairy Cattle of Different Lactation Stages[J];黑龙江八一农垦大学学报;2015-03
2 ZHANG Zong-wei;LI Na;LI Zhao-hua;ZHANG Shu-min;ZHANG Min;Agricultural College of Yanbian University;Sciences Academy of Jilin Province;;The influence of monascus with Chinese medicine on the performance of pig growth[J];延边大学农学学报;2013-03
3 LIU Huan,LI De-yun(Agricultural College of Yanbian University,Yanji jilin 133002,China);Effect of different content of Moisture TMR on cellulolytic enzyme activity of rumen and microbial ecology[J];延边大学农学学报;2013-02
4 WANG Li-zhe,LEI Lian-cheng,HAN Wen-yu (College of Animal Science and Veterinary Medicine ,Jilin University,Changchun 130062,China);Construction of Prokaryotic Coexpression Vector for Lysis Gene E and Staphylococcus Nuclease Gene and Preparation of E. coli Bacterial Ghost[J];中国生物制品学杂志;2007-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Chang Chunlong;Wang Shixia;Zhai Junjun;Ni Hongbo;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;;Preparation of Cattle Pasteurella multocida Ghosts[J];黑龙江八一农垦大学学报;2017-03
2 Wang Gang;Shu Shi;Jin Xishan;Xia Cheng;Wu Ling;Yang Wei;Xu Chuchu;Xiao Xinhuan;Bai Yunlong;Fan Ziling;Xu Chengbin;Weng Chunling;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;Daqing Animal husbandry and Fisheries Bureau;Daqing Animal Breeding Center;;Control Effect of Hypocalcemia of Dairy Cattle in Intensive Dairy Farm[J];黑龙江八一农垦大学学报;2016-06
3 Cao Yu;Li Lan;Zhang Hongyou;Collge of Animal Science and Technology,Heilongjiang Bayi Agricultural University;;Study of Blood Clinical Pathological Changes of Selenium Deficiency of Dairy Cattle in Intensive Dairy Farm[J];黑龙江八一农垦大学学报;2016-06
4 GUO Yunxia;HAO Qinghong;LIU Yueqin;ZHANG Yingjie;REN Shuyue;College of Animal Science and Technology,Hebei Agricultural University;College of Life Science ,Hebei Agricultural University;;Effects of Microbial Fermentation Corn Straw on Rumen Fermentation and Microbial Diversity of Dorset×Thin-Tailed Han Weaned Lambs[J];动物营养学报;2017-01
5 Xu Chuang;Yu Xin;Zhang Qi;He Yibo;Li Lingzheng;Zhao Xiangyang;Chen Shuang;Dong Zhihao;Huang Baoyin;Wu Zhengjiao;Liu Mingxing;Chen Yuanyuan;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;;Correlation Analysis of Fibroblast Growth Factor 21 Levels and β-hydroxybutyric Acid in Dairy Cattle Serum with Subclinical Ketosis[J];黑龙江八一农垦大学学报;2016-03
6 Zhou Yaqiang;Han Yahui;Jiang Zaihui;Ma Yanxuan;Shi Shaolei;Sun Ruitao;Li Yue;Han Hua;Tang Dejiang;Hou Jianjun;Cao Yang;College of Animal science and Technology,Heilongjiang Bayi Agricultural University;Heilongjiang Zhongsheng Husbandry Co.Ltd.;;Effects of Adding Lactic Acid Bacteria and Molasses on Fermentation Characteristics and in Vitro Ruminal Methane Production of Corn Stover Silage[J];黑龙江八一农垦大学学报;2016-03
7 YAN Yibo;ZHANG Yuhuan;;Research advance of application of TMR technique in mutton sheep production[J];中国饲料;2016-02
8 ZHENG Bowen;ZHANG Jinji;LI Deyun;Agricultural College of Yanbian University;;Effect of straw of different length TMR on fiber decomposition enzyme activity of rumen and rumen ecology in sheep[J];延边大学农学学报;2015-04
9 ZHANG Yu-min;ZHANG Jin-ling;LIU Wen-hua;College of Animal Science and Technology,Qingdao Agricultural University;Weifang Exit-entry Inspection and Quarantine Bureau;;Preparation Methods and Applications of Bacterial Ghosts[J];动物医学进展;2014-11
10 WU Yi-chun(College of Biological Engineering,Binzhou Polytechnic,Binzhou 256603,China);Production and lysis efficiency of pig pathogenic Escherichia coli O_(139) ghost[J];中国兽医科学;2013-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHU Wen;LIU Jian-xin;YE Jun-an;Institute of Dairy Science,Zhejiang University;;Research Advances in the Application of Zinc in Dairy Cows[J];中国畜牧杂志;2014-15
2 Song Liguo;Qi Kai;Zhang Ying;Wang Wentao;Wang Chunren;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;Animal Health Supervision,Qiqihar Land Reclamation Region of Heilongjiang;Yanbian Animal Husbandry of Jilin;;Analysis on Business Model and Economic Efficiency of Cow Borough in Qiqihar Land Reclamation Region of Heilongjiang[J];黑龙江八一农垦大学学报;2014-03
3 Wang Yang;Qu Yongli;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;;Research Progress of Nutrient Requirements for Replacement Heifers in Different Growing Stages[J];黑龙江八一农垦大学学报;2014-01
4 ZHENG Jia-san,XIA Cheng,WANG Lin-lin,ZHANG Hong-you,XU Chuang,WU Ling(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China);Effect of Rumen-protected Vitamin D on Hypocalcaemia of Dairy Cows in Transition Period[J];中国畜牧兽医;2013-08
5 JIN Hua-lian,DONG Yu-ying,ZHANG Min,LIANG Cheng-yun(Agricultural College of Yanbian University,Yanji Jilin 133002,China);Effect of Monascus Chinese medical compound preparation on production performance of laying hens and eggs conventional quality[J];延边大学农学学报;2012-04
6 TAN Jian-hua1,2,YUAN Jiu1,2,LI Fa-di1,2,LI Chong1,2,MA You-ji1,2,ZHENG Chen1,2, WANG Xiao-juan1,2,GUO Jiang-peng1,2,YU Jie1,2,GAO Tian-xi3,CHEN Huang-he1(1.College of Animal Science & Technology,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Feed Engineering Technology Research Center,Lanzhou 73000,China;3.Minqin Longyuanzhongtian Biological Engineering Co.Ltd,Minqin 733300,China);Effects of nutrition levels and particle sizes of total mixed ration on rumen metabolic parameters of sheep[J];甘肃农业大学学报;2012-01
7 ZHANG Hong-gang1,SHI Wei-ping2,WANG Fu-zhong3(1.Institute of Animal Husbandry & Veterinary,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China; 2.Taiyuan College of Animal Husbandry and Veterinary,Shanxi Agricultural University,Taiyuan 030024,China; 3.Qingxu Fu-xing-da Animal Farming Cooperatives,Qingxu 030401,China);Granular Total Mixed Rations on the Performance of Confined Lambs[J];山西农业科学;2011-05
8 NIE Bing et al(Agricultural College,Yanbian University,Longjing,Jilin 133400);Effects of Monascus and Chinese Traditional Medicine Synbiotics on the Conventional Indices of Mice[J];安徽农业科学;2009-06
9 ZHANG Rui-Ping, ZHANG Zhao-Shan** (Institute of Biotechnology, Academy of Military Medical Sciences, Beijing100071,China);Advances on The Novel Drug Deliver System:Bacterial Ghost[J];生物化学与生物物理进展;2006-07
10 GAO Zheng-qin, XING Hua, SUN Huai-chang, LI Hou-da (Comparative Medicine Center, Yangzhou University, Yangzhou 225009, China);Cloning and Sequencing of the nuc Gene of Staphylococcus aureus[J];上海实验动物科学;2003-01
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