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《Journal of Heilongjiang Bayi Agricultural University》 2017-03
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Effect of Inhibitors on the Lycopene Production from Blakeslea trispora

Cai Wenjie;Tang Huacheng;Wen Danxu;College of Food Science,Heilongjiang Bayi Agricultural University;  
In order to select the high efficient and safe blocking agent,comparative test method was used to study Blakeslea trispora metabolic synthesis of lycopene,beta-carotene and bacteria amount of biomass,choose the tea-leaf of jasmine tea,theophylline and nicotinamide as the inhibitors,and further the conditions that tea as a blocker produce lycopene were optimized.The results showed that:tea-leaf of jasmine tea,theophylline,nicotinamide could promote Blakeslea trispora to accumulates lycopene. After 40 h of fermentation adding 9 g·L~(-1)theophylline,8 g·L~(-1) tea-leaf of jasmine tea,6 g·L~(-1) nicotinamide,lycopene synthesis were 36.32 mg·L~(-1),158.38 mg·L~(-1),34.69 mg·L~(-1),respectively. The beta carotene contents were 33.25 mg·L~(-1),42.07 mg·L~(-1),31.79 mg·L~(-1),Bacteria biomass was 24.69 mg·L~(-1),38.53 mg·L~(-1),27.40 mg·L~(-1). In which tea-leaf of jasmine tea was the best inhibitor,the further experiments showed that the best adding time was 48 h,optimum adding quantity was 9 g·L~(-1),lycopene biosynthesis could reach205.49 mg·L~(-1).The research results provided a theoretical reference for the study of the metabolic pathway of the lycopene into carotene.
【Fund】: 基金项目:三孢布拉霉菌发酵生产番茄红素的最佳条件的筛选(GZ11B401)
【CateGory Index】: Q936
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