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《Journal of Heilongjiang Bayi Agricultural University》 2017-04
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Establishment of Hypoxia Stress Model Using Cobalt Dichloride in HepG2 Cells

Chang Xiaocui;Fan Weifeng;Shen Huanhuan;Huang Xiangyue;Wang Sheng;Wang Yuan;Ran Wei;Sun Kexin;Jiang Nan;Kong Fanzhi;College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University;Laboratory Department,People's Hospital of Daqing;  
To find a proper condition to establish hypoxia stress model using cobalt dichloride in HepG2 cells and evaluate it.After using different concentration of CoCl_2 to treat HepG2 cells,expression of AK4,ATF4 and HIF-1α was detected by PCR;compare cell proliferation rate by cell counting. With the increase of CoCl_2,the expression of HIF-1α m RNA and ATF4 m RNA were both increased,however,AK4 m RNA were decreased significantly;cell proliferation rate was decreased significantly(P 0.05)and the membrane was broken. The expression of HIF-1α,which was the most important transcription factor in hypoxia stress,were increased in HepG2 cells during CoCl_2 treatment,so,it was feasible to establish hypoxia stress model by using CoCl_2. ATF4 and AK4 may play an important role in protecting HepG2 cells from hypoxia stress. Establishment of this model may lay a foundation for further research on AK4 physiology function.
【Fund】: 黑龙江八一农垦大学大学生创新创业训练计划项目
【CateGory Index】: S852.3
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