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《Journal of Heilongjiang Bayi Agricultural University》 2017-04
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Preparation and Application of Polyclonal Antibody against Porcine Integrin αv

Wang Enyu;Gao Jing;Guo Donghua;Li Chunqiu;Sun Dongbo;College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University;  
Construction of prokaryotic expression vector of porcine integrin αv gene,purification of fusion protein which was used to immunize mice to prepare and then to identify and apply the polyclonal antibody against porcine integrin αv gene.The extracellular domain and antigenicity gene sequence of porcine integrin αv was truncated after prediction and analysis.The gene sequence was synthesized according to optimize the Escherichia coli rare codon and cloned into the p GEX-6p-1 prokaryotic expression vector.The recombinant protein was expressed and purified and then identified by western blot which was immunized mice to prepare polyclonal antibody.The antibody titer was used indirect ELISIA method to detect,and then detect the response of natural integrin αv protein by Western blot.The 83 ku molecular weight recombinant protein was obtained.The polyclonal antibody titer was more than 1 ∶6 400 which was prepared by the purified of recombinant protein and it was able to react with native integrin αv protein.The polyclonal antibody against porcine integrin αv was prepared which provided some basis for following further related research.
【Fund】: 国家自然科学基金面上项目(31472209)
【CateGory Index】: S852.4
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