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《Journal of Heilongjiang Bayi Agricultural University》 2017-04
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Identification of Morphology and Molecular Biology of Cylicocyclusnassatus

Gao Yuan;Qiu Jianhua;Chang Qiaocheng;Gao Junfeng;Wang Chunren;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;  
In order to confirm the species of the nematodes,the worms from the naturally infected horse in Daqing were identified based on the morphological observation and molecular biological method.The result of the morphological observation showed that the worms mightbe the Cylicocyclusnassatus.The nuclear ribosomal internal transcribed spacer sequences(ITS)of single female and male worms were amplified by PCR.The results showed that the length of the ITS sequences were all 842 bp,and the length of the ITS1,5.8S and ITS2 were 370 bp,152 bp and 320 bp,respectively.Compared the ITS sequences of female and male worms with the C.nassatus available in the Gen Bank,the sequences identity were 99.8% and 99.6%,respectively.So the result demonstrated that the nematodes were C.nassatus.
【Fund】: 黑龙江省普通高等学校黑龙江八一农垦大学动物医学重点实验室开放课题(AMKL201304)
【CateGory Index】: S852.7
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