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《Journal of Heilongjiang Bayi Agricultural University》 2017-04
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Stability of the Alternaria mali Roberts Exopolysaccharides and the Change from Spectrophotometer to Multiskan Spectrum

Fang Fang;Liu Chunyan;Chen Kaoshan;Yuan Pingchuan;Wang Hao;Wannan Medical College;Shandong University;  
In order to find an efficient,quick and stable method for determination of Alternaria mali Roberts exopolysaccharides,the content and stability of exopolysaccharides in light condition,different temperature,pH,metal ions were studied by a multiskan spectrum microplate spectrophotometer. The results showed that the method for mensurating the exopolysaccharide content by multiskan spectrum had good linear relationship,stability and repeatability,the operation was simple and suitable for large sample measurement and analysis. The Alternaria mali Roberts exopolysaccharides had better thermal stability at 20-80 ℃ and remained stable in weak acid(pH=6) and Na+ ion solution,strong acid and alkali solution or Cu~(2+)、Fe~(3+) ions had an impact on the stability of exopolysaccharides,the light could also influence the stability.
【Fund】: 安徽省自然科学基金(1408085MH197);; 安徽省教育厅重点项目(KJ2015A199);; 大学生科研资助金(WK2015S23)
【CateGory Index】: Q936
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