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《Heilongjiang Agricultural Sciences》 2017-10
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Application of the Optimized Expression of the Zearalenone Degrading Enzyme

LIU Meng-ran;College of Life Science,Northeast Agricultural University;  
In order to increase the expression quantities of ZEN degrading enzyme,the zearalenone degradation enzyme genes have been amplified by using the PCR method from a zearalenone degradation enzyme sequence and constructed the Aspergillus niger expression vector pSZHG-ZEN,vector pSZHG-faeA-ZEN with endogenous fusion protein high expression of ferulic acid esterase gene.The maximal fermentation enzyme activity of pSZHG-ZEN and pSZHG-faeA-ZEN were 383.77 and 1 378.33 U·mL~(-1),it can be inferred that the expression vector of fusion protein has higher vitality.
【CateGory Index】: Q78;Q936
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