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《Heilongjiang Agricultural Sciences》 2017-10
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Isolation and Growth Conditions of Termitomyces albuminosus

LI Yan-li;DENG Bai-wan;HUANG Man-man;XIE Xiu-chao;PENG Hao;School of Biological Science and Engineering,Shaanxi University of Technology;Shaanxi Engineering Research Center of Edible and Medicated Fungi;  
To solve the difficult problem of separating Termitomyces albuminosus,the isolation,identification and growth characteristics of Qinling Mountains area from a wild Termitomyces albuminosus were studied,tissue isolation method was used to isolate and purify the strains,its genus was determined by plate observation and ITS sequence analysis,the optimum growth carbon source,nitrogen source,KH_2PO_4 concentration,MgSO_4 concentration,temperature and pH were investigated with the growth rate of mycelium as index.The results showed that according to the morphological characteristics and ITS sequence analysis to determine the strain of Termitomyces albuminosus,the optimum carbon source for the growth of mycelia was corn flour;the optimum nitrogen source was ammonium nitrate;the concentration of KH_2PO_4 was 0.10%~0.20%,the optimum concentration was 0.15%;the concentration of MgSO_4 was 0.025% ~0.075%,the optimum concentration was0.050%;the growth temperature range was 25~27 ℃,the optimum temperature was 26 ℃;the growth pH range was 5.0~6.0,the optimum pH was 5.0.
【Fund】: 2016年科技厅科技创新工程资助项目(2016HBGC-07)
【CateGory Index】: S646
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