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《Heilongjiang Agricultural Sciences》 2017-10
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Extraction Method of Polysaccharides from Organic Auricularia auricula of Changbai Mountain

LIN Hua;CHE Cheng-lai;WANG Xia;WANG Xin-yu;WANG Yu-hui;XUAN Dong-zhe;JIN Long-zhe;Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences;  
To explore the extraction methods of microwave assisted extraction,optimizing the conditions on polysaccharides from organic Auricularia auricula of Changbai mountain,finally to determine the best extraction method.The results of single factor test and orthogonal test proved that better extration conditions were water as extraction agent,microwave power 800 W,ratio of material to liquid 1∶40,extraction temperature 95 ℃,the microwave radiation time 40 minutes,water bath extraction time was two times,and the time was 1.5 hours each time,water content were 400 and 500 mL,under these coditions,the extraction rate of polysaccharide was19.30%.After removing protein by tannic acid method of precipitation,the content of polysaccharides in Auricularia auricula was 44.5%.
【Fund】: 延边州科技发展计划资助项目(2014AC29)
【CateGory Index】: S646.6
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