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《Heilongjiang Animal Science and Veterinary Medicine》 2010-01
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Thacheal epithelium lesion of chickens vaccinated with recombinant NDV La Sota vaccine strain expressing HA gene of H5 highly pathogenicity Avian influenza virus

DONG Jun-bin1,2,MA Xue-en1,BU Zhi-gao2(1.The College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)  
Vaccination was given by eyedrop and intranasally instillation with rLa Sota-HA(GD)(2×106 EID50/chicken)to two-day-old SPF chicken after 1,3,5,10,15,18 days.The tracheas were collected.Ultrastructural changes in the tracheal epithelium were examined by scanning electron microscopy.On day 1 postvaccation(PV)scanning electron microscopy revealed hypertrophy of goblet cell and small patches of the deciliated epithelium around the disoriented and deformed cilla.On days 1 PV hyperplasia of goblet cells accompanied by an increase in mucus.On days 1 PV globular particles found in areas of the trachea epithelium with tips of cilia adhering to them,goblet cells and basal cell regenerated.Non-ciliated plaques were observed until day 18 PV.But they gradually decreased in size.Light microscopy showed small vacuoles containing lymphocytes and heterophils plasma cells in the epithelial layer.The submucosa is slightly edematous.immature epithelium proliferated in some areas.The trachea is lined with several layers of immuture cells on days 3 PV.There is hyperplasia of mucous gland cells causing narrowong or obliteration of glandular lumina in immunohistochemical(IHC) staining on days 10 PV.Specilfic staining was localized in the submucosa cells.This response is probably an attempt by the mucocillary defense mechanism.
【Fund】: “973”国家重点基础研究计划项目(2005CB523200)
【CateGory Index】: S852.659.5
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