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《Heilongjiang Animal Science and Veterinary Medicine》 2010-01
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Study on the resistant mechanism of Streptococcus suis to tetracycline in Northeast China

LU Jia,LI Yi-jing(College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China)  
Purpose of the experiment was to analyse the molecular mechanism of Streptococcus suis which had been in Northeast area resist tetracycline.On the base of lancefield grouping,twelve strains of Streptococcus suis were detected the resistance to tetracycline and were amplified correlative genes by PCR.The results showed that twelve strains had belonged to group D of lancefield.And 91.7% of strains were resistance to Doxycycline,100% to Hydroxytetracycline,83.3% to Chlortetracycline,twelve strains were all amplified tetM gene by PCR,but none of strains harbored tetO gene.Nucleotide sequence comparison showed that the tetM gene had 94%~100% nucleotide sequence identities with the published sequences of Streptococcus pneumoniae,Enterococcus faecalis in GenBank.
【Fund】: “十五”国家科技攻关计划项目(2004BA519A60)
【CateGory Index】: S852.61
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