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《Heilongjiang Animal Science and Veterinary Medicine》 2010-01
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Factors affecting the isolation and culture of embryonic stem cell in KM mice

HUANG Zhi-jun,LV Zhong-hua,ZHENG Peng,LI Dong-xu,ZHANG Gui-xue(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China)  
The experiment was designed to study the influencing factors of isolation and culture of KM mouse embryonic stem cell(ESC).In this experiment,conditioned medium was made by cadiocyte from 3~5 d rat for the first time,effect of four culture system and different feeder layers on isolation and culture of ESC were studied.The result showed that culture medium adding LIF or RH-CM had a significant difference in adherent ratio,ICM formation ratio,primary ESC formation and inhibiting differentiation comparing with the culture medium without LIF or RH-CM,feeder layer made by MEF had a better effect of isolation and culture of ESC than feeder layer made by mouse sertoli cells.
【Fund】: 黑龙江省农业科学院博士后基金项目(LRB04-185)
【CateGory Index】: S814
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Chinese Journal Full-text Database 1 Hits
1 HAN Rong\ CHAI Guixuan\ SHANG Kegang (College of Life Sciences, Peking University, Beijing, 100871);The Effect of Rat Heart Conditioned Medium in Forming Mouse ES Cell Colonies[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1997-02
Chinese Journal Full-text Database 10 Hits
1 WANG Yi-min et al(College of Animal Medicine,Northwest A&F University,Yangling,Shaanxi 712100);The Effect of Different Culture System and Different Passage Methods on the Isolation and Culture of Embryonic Germ Cells from Kunming White Mouse[J];Journal of Anhui Agricultural Sciences;2008-12
2 YU Shu-Min~(①②) XU Xiao-Ming~① HUA Jin-Lian~① DOU Zhong-Ying~①(① The Research Centre of Stem Cell Engineering and Technology,Northwest SciTech University of Agriculture and Forestry,Yangling 712100;② Animal Sci-Tech College,Sichuan Agricultural University,Ya′an 625000,China);Mouse Embryonic Stem Cells: Advances in Technology[J];Chinese Journal of Zoology;2006-01
3 ZHOU Zhen ming, SAN Run zi(College of Animal Science and Technology, Hebei Agricultural University, Baoding, Hebei 071001, China);Research Progresses of the Factors Affecting the Separation and Cloning of Embryo Stem Cell[J];Journal of Yellow Cattle Science;2001-04
4 Meng Chaoheng Li Zongqiang Liang Mingzhen(Animal Reproduction Institute,Guangxi University,13 Xiulinglu,Nanning,Guangxi,530005,China);On Culture Systems of Embryonic Stem Cells[J];Guangxi Sciences;2002-04
5 LIU Jun-ping~(1,2),CAO Hong-guo~1,AN Zhi-xing~1,ZHAO Hui-ying~1,WEN Ye-fei~1,ZHANG Yong~1(1 Institute of Bio-engineering,Northwest A & F University,Yangling,Shaanxi 712100,China;2 College of Animal Science and Animal Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018,China);Study on culture characteristics of rat cardiomyocytes in vitro[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2005-01
6 LIU Jun-ping~(1,2),CAO Hong-guo~1,ZHENG Yue-mao~1,ZHAO Hui-ying~1,WEN Ye-fei~1,ZHANG Yong~1(1 Institute of Bio-Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China;2 College of Animal Science and Animal Medicine,Inner Mongolia Agriculture University,Hohhot,Inner Mongolia 010018,China);Effect on isolation and culture of Kunming white mouse ES cells with media conditioned of rat cardiomyocyte[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2005-02
7 CAO Hong-guo, ZHANG Yong(College of Animal Science and Technology, Northwest Science-Technology University of Agriculture and Forestry, Yangling 712100, China);Somatic Cell Nuclear Transfer with Skin Fibroblasts as Donor Cells in Mouse[J];Chinese Journal of Animal and Veterinary Sciences;2005-06
8 MENG Guo liang,TANG Fu chou,TENG Lu,SHANG Ke gang (College of Life Science , Peking University,Beijing 100871,China);The Problems and Solution Methods in Establishing and Maintaining ES Cell Lines[J];Hereditas(Beijing);2001-03
9 Huang Bing Huang Wen ge Zhong Nu qi Chen Ying qing Chen Xi gu (Experimental Animal Center, Sun Yet-sen University of Medical Sciences, Guangzhou, 510089, China);A Study on Six Kinds of Cell Culture System for Mouse Embryonic Stem Cells[J];ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA;2000-01
10 Liu Junping 1,2, Cao Hongguo 1, An Zhixing 1, Zhang Yong 1 (1Institute of Bio-engineering, Northwest A & F University, Yangling 712100;2College of animal science and animal medicine, Inner Mongolia Agricultural University, Hohhot, 010018);Comparison to Isolation of Mouse ES Cells With LIF Media and Cardiomyocyte Media Conditioned[J];Chinese Agricultural Science Bulletin;2005-03
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