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《Heilongjiang Animal Science and Veterinary Medicine》 2010-01
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Factors affecting the isolation and culture of embryonic stem cell in KM mice

HUANG Zhi-jun,LV Zhong-hua,ZHENG Peng,LI Dong-xu,ZHANG Gui-xue(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China)  
The experiment was designed to study the influencing factors of isolation and culture of KM mouse embryonic stem cell(ESC).In this experiment,conditioned medium was made by cadiocyte from 3~5 d rat for the first time,effect of four culture system and different feeder layers on isolation and culture of ESC were studied.The result showed that culture medium adding LIF or RH-CM had a significant difference in adherent ratio,ICM formation ratio,primary ESC formation and inhibiting differentiation comparing with the culture medium without LIF or RH-CM,feeder layer made by MEF had a better effect of isolation and culture of ESC than feeder layer made by mouse sertoli cells.
【Fund】: 黑龙江省农业科学院博士后基金项目(LRB04-185)
【CateGory Index】: S814
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【Citations】
Chinese Journal Full-text Database 1 Hits
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
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