Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Heilongjiang Animal Science and Veterinary Medicine》 2010-23
Add to Favorite Get Latest Update

A study of PCR assay for detection of pseudorabies based on PRV gE gene

YANG Xue,ZHU Ling,SHI Xiao-hong,GUO Wan-zhu(Animal Biotechnology Center and Laboratory of Animal Disease and Human Health,Sichuan Agricultural University,YA'an 625014,China)  
To establish a method for rapid diagnosis of pseudorabies,the experiment designed a pair of specific primers based on the gE gene sequence of PRV Min-A strains(accession number: AY170318.1) which was published in the NCBI PRV gene was amplified by using PCR technology,testing the PCR detection method established by specificity,sensibility,reproducibility.The results indicated that the size of target gene was 348 bp by PCR amplification.Negative test results were found on the porcine reproductive and respiratory syndrome virus,porcine circovirus type 2,classical swine fever virus and porcine parvovirus.As little as 10 pg of PRV DNA could be detected with high reproducibility.The PCR method were tested by 43 clinic suspicious samples,and 65.12%(28/43) of the samples were pointed out to be positive.This illustrateds that the PCR method can applied for the clinical investigation and epidemiological survey of brucellosis.
【Fund】: “十一五”国家科技支撑计划项目(2006BAD06A18);; “长江学者和创新团队发展计划”创新团队项目(IRT0848)
【CateGory Index】: S858.28
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Lei1,2,GUAN Yu1,2,LI Kun-lin1,YU Xin-you2,SHEN Zhi-qiang1,2(1.Shandong Binzhou Animal Science and Veterinary Medicine Academy,Binzhou 256600,China; 2.Shandong Lüdu Bio-Science and Technology Co.Ltd.,Binzhou 256600,China);Research advance in molecular biology of pseudorabies virus[J];Chinese Veterinary Science;2011-07
【Citations】
Chinese Journal Full-text Database 3 Hits
1 JIAO Ming~(1,2) ZHAO Yu-jun~1 ZHUANG Jin-qiu~2 SHEN Zhi-qiang~2 1.College of Animal Science and Veterinary Medicine,Shenyang Agriculture University,Shenyang,Liaoning,110161,China; 2.Institute of Animal Husbandry and Veterinary Medicine,Binzhou Academy of Shandong,Binzhou,Shandong,256600,China;Progress on the Diagnostic Method of Porcine Pseudorabies[J];Progress in Veterinary Medicine;2007-01
2 LOU Gao ming,DU Wei xian,LIAO Xiao Ping,XIE Ming quan (The Guangdong Key Laboratory of Veterinary Biotechnology,Guangzhou 510640,China);Detection of Pseudorabies Virus DNA by PCR[J];Chinese Journal of Biochemistry and Molecular Biology;2001-04
3 ZHOU Bin, SU Xin-ming,ZHANG Su-fang,JIA Yun,CAO Rui-bing,CHEN Pu-yan** (Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture of People’Republic of China, Nanjing Agricultural University, Nanjing 210095,China);Establishment and Application of PCR to Detect Wild-Type Pseudorabies Virus[J];Virologica Sinica;2004-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Huan-chun, ZHOU Fu-chun, FANG Liu-rong, HE Qi-gai, WU Bin, HONG Wen-zhou (Lab of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China);Construction of TK~-/gG~-/LacZ~+ Mutant of Pseudorabies Virus Strain Ea[J];Chinese Journal of Virology;2001-01
2 ZHANG Li,DING Bo-liang,WANG Ying-zhen,YAN Ming-hua(Tianjin Institute of Animal Husbandry and Veterinary Science,Tianjin,300112,China);A gE-PCR for Differentiation Pseudorabies virus gE-Vaccine Strain from Wild-type Strains[J];Progress in Veterinary Medicine;2008-11
3 LIU Yuan-yuan1,WU Hui1,XIAO Xing-long1,2,JIA Yun3,WU Bin3,YU Yi-gang1(1.College of Light Industry & Food,South China University of Technology,Guangzhou 510640,China;2.Shenzhen Taitai Genomics,Inc.,Shenzhen 518057,China;3.Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116001,China);Establishment and Application of Fluorescence Quantitative TaqMan-MGB PCR Assay for Pseudorabies Virus[J];China Animal Husbandry & Veterinary Medicine;2009-04
4 LI Ming-feng1,2,WEI Zhan-yong1,2,GUO Xian-po1,2,JIA Yan-yan2,CHEN Hong-ying1,WANG Xue-bing1,CUI Bao-an1,2(1.Engineering College of Animal Husbandry And Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Animal Food Safety Key Labrotorary,Zhengzhou 450002,China);Development of SYBR-GreenⅠ real-time quantitative PCR assay for detection of pseudorabies virus[J];Journal of Henan Agricultural University;2009-03
5 CHEN Zhen-hai, YANG Han-chun**, GUO Xin, CHEN Yan-hong (Key Laboratory of Preventive Veterinary Medicine, Ministry of Agriculture,China Agricultural University,Beijing 100094, China);Construction of the Recombinant Adenovirus Expressing gC Glycoprotein Gene for Developing Vaccine against Pseudorabies virus[J];Journal of Agricultural Biotechnology;2007-02
6 Guo Wanzhu (Sichuan Agricultural University,Yaan,Sichuan,China);DETECTION OF PSEUDORABIES VIRUS BY ~(32)p LABELED DNA PROBE[J];Journal of Sichuan Agricultural University;1991-01
7 Li Zhao1, 2, Baoan Cui1, Hongying Chen1, Zhanyong Wei1, Lanlan Zheng1, Xiaoli Lü1, Yanyan Jia1, and Xuyong Zhao2 1 College of Animal Husbandry And Veterinary, Henan Agricultural University, Zhengzhou 450002, China; 2 College of Zhengzhou Animal Husbandry Engineering, Zhengzhou 450011, China;Diagonsis Establishment of Fluorescen Quantitative PCR Assay for Pseudorabies Wild-type Virus and Vaccine Virus[J];Chinese Journal of Biotechnology;2008-07
8 XU Gao- yuan,WANG Xiang,CHEN Huan- chun,XU Xiao- juan,Li Zi- li,HE Qi- gai,WU Bin (College of Veterinary Medicine of Huazhong Agricultural University,Wuhan 430070,China);Construction of Recombinant Virus Strain TK~- /gG~- /NS1~+ Recombinized by Pseudorabies Virus Ea Mutant Strain TK~- /gG~- /LacZ~+ and NS1 Gene of Japanese Encephalitis Virus[J];Chinese Journal of Animal and Veterinary Sciences;2004-02
9 LIU Li-na, HE Qi-gai, CHEN Huan-chun, LIU Zheng-fei, ZHANG Song-lin, WANG Zhao-xiong (National Key Laboratory of Agricultural Microbiology/Huazhong Agricultural University, Wuhan 430070, China);Detection of porcine pseudorabies virus and differentiation of natural PRV from vaccinal PRV by multi-PCR[J];Chinese Journal of Veterinary Science and Technology;2005-02
10 CHEN Lu~(1,2), ZHA Guang-ming~(1), WANG Chuan-qing~(1), GUO Wan-zhu~(2), XU Zhi-wen~2(1. College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002,China;2. Center of Animal Biotechnology, Sichuan Agricultural University, Ya’an 625014,China);Development of PCR for identifying pseudorabies virus gE/gB strains and its application[J];Chinese Journal of Veterinary Science and Technology;2005-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 NIU Chun-ling,QI Xiao-le,CUI Bao-an,WANG Xin (Laboratory of Agricultural Biological Technique,Henan Agricultural University,Zhengzhou,Henan,450002,China);Application of ELISA in the Diagnosis on The swine Pseudorabies[J];Progress In Veterinary Medicine;2004-03
2 LIU Jian-zhu,CUI Yu-dong,ZHU Zhan-bo,PIAO Fan-ze(College of Animal Science and Technology, Heilongjiang August First Agricultural University ,Daqing, Heilongjiang,163318,China);Progress of the Diagnostic Method of Porcine pseudorabies[J];Progress In Veterinary Medicine;2004-05
3 CHEN Lei, ZHANG Lu-an ,LI Yan ,RAN Duo-liang(Xinjiang Agriculture University ,Wulumuqi , Xinjiang, 830063, China; Xinjiang Animal Husbandry and Veterinary Station,Wulumuqi, Xinjiang,830063,China);The Progress of Swine pseudorabies[J];Progress In Veterinary Medicine;2004-05
4 HUANG Wei jian 1,2 , JIANG Yan 1,CHEN De sheng 1, LU Yin hua 1, ZHANG Xue lian 1, CHEN Pu yan 1 (1.Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture, Nanjing Agric Univ,Nanjing 210095,China;2.College of Animal Science and Technology, Guangxi Univ,Nanning 530005, China);Cloning, sequence analysis of the gG gene of Pseudorabies virus SH strain[J];Journal of Nanjing Agricultural University;2003-01
5 LOU Gao ming,DU Wei xian,LIAO Xiao Ping,XIE Ming quan (The Guangdong Key Laboratory of Veterinary Biotechnology,Guangzhou 510640,China);Detection of Pseudorabies Virus DNA by PCR[J];Chinese Journal of Biochemistry and Molecular Biology;2001-04
6 TANG Yong~(1,2), CHEN Huan-chun~1, QIN Ya-li~1, FANG Bing-bing~1, HE Qi-gai~1, JIN Mei-lin~1, WU Bin~1, LIU Zheng-fei~1 (1.Lab of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China; 2.Department of Bioengineering, Jinan University, Guangzhou 510632, China);The Development and Application of the gG Enzyme Linked Immunosorbent Assay for Detection of the Antibody to gG Protein of Pseudorabies Virus in Porcine[J];Chinese Journal of Animal and Veterinary Sciences;2004-05
7 LIU Li-na, HE Qi-gai, CHEN Huan-chun, LIU Zheng-fei, ZHANG Song-lin, WANG Zhao-xiong (National Key Laboratory of Agricultural Microbiology/Huazhong Agricultural University, Wuhan 430070, China);Detection of porcine pseudorabies virus and differentiation of natural PRV from vaccinal PRV by multi-PCR[J];Chinese Journal of Veterinary Science and Technology;2005-02
8 CHEN Lu~(1,2), ZHA Guang-ming~(1), WANG Chuan-qing~(1), GUO Wan-zhu~(2), XU Zhi-wen~2(1. College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002,China;2. Center of Animal Biotechnology, Sichuan Agricultural University, Ya’an 625014,China);Development of PCR for identifying pseudorabies virus gE/gB strains and its application[J];Chinese Journal of Veterinary Science and Technology;2005-05
9 Shi Jianping et al(Veterinary College,Changchun University of Agriculture and Animal Sciences);Detection of Pseudorabies Virus DNA from Infected Cell Culture and Tissue Samples by Polymerase Chain Reaction[J];;1996-05
10 QIU Dexin,CHEN Huanchun,HE Qigai,FANG Liurong,WU Bin,ZHOU Rui (Animal Virus Lab of Animal Science and Veterinary Medicine College of Huazhong Agricultural University,Wuhan Hubei 430070);Comparison of Latex Agglutination Test (LAT) and Serum Neutralization Test (SNT) for Detecting Serum Antibody Against Porcine Pseudorabies Virus(PRV)[J];Chinese Journal of Preventive Veterinary Medicine;2000-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved