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《Heilongjiang Animal Science and Veterinary Medicine》 2017-05
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Experimental study on the anti-inflammatory effect of aqueous extract from Venenum bufonis

LIANG Zhengmin;WEI Yingyi;PENG Jianbo;HUANG Hongye;NIE Haiying;XU Yangfeng;HE Jiakang;College of Animal Science and Technology,Guangxi University;Guangxi Engineering Research Center for Veterinary Medicine;  
To evaluate the anti-inflammatory effect of aqueous extract from Venenum bufonis in vitro,a cellular inflammation model was established by LPS inducing RAW 264. 7 cells and mouse peritoneal macrophages. The Griess method was used to determine the effect of aqueous extract from Venenum bufonis on the level of nitric oxide( NO) in the cell supernatant,and the contents of TNF-α,IL-1β and IL-6 were measured by ELISA assay,and the intracellular reactive oxygen species( ROS) level was measured by a fluorescence method. The results showed that the aqueous extract from Venenum bufonis had significantly inhibited effects on the secretion of inflammatory factors including NO,TNF-α,IL-1β and IL-6 in RAW 264. 7 cells and mouse peritoneal macrophages induced by LPS,which was significantly reduced compared with the LPS group. Meanwhile,the aqueous extract from Venenum bufonis could extremely significantly reduce( P 0. 01) the intracellular ROS level. The results indicate that the aqueous extract from Venenum bufonis may reduce the intracellular ROS level and alleviate the cellular inflammatory response by inhibiting inflammatory factors,showing a good anti-inflammatory effect.
【Fund】: 国家自然科学基金项目(31360624);; 广西自然科学基金项目(2014GXNSFAA118115);; 广西大学科研基金项目(XBZ120918)
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 5 Hits
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【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 ZUO Qi-yan;ZHAO Yan-min;LI Zhen-lin;QIAN Shi-hui;PU She-ban;State Key Laboratory of Natural Medicines,Research Department of Pharmacognosy, China Pharmaceutical University;Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine;Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Jiangsu Province;;The Research Progress of Traditional Chinese Medicine Bufonis Venenum[J];人参研究;2017-03
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