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《Heilongjiang Animal Science and Veterinary Medicine》 2017-08
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Recovery technology of waste ethanol after HE staining of paraffin sections

LIAO Qiuping;JING Bo;WANG Yanping;DENG Yuping;ZHOU Yanqing;NIU Youlong;LI Lianrui;College of Animal Science,Tarim University;  
Hematoxylin eosin staining,abbreviated as HE staining,is widely used in scientific experiments. The conventional HE staining requires a large amount of gradient ethanol solution,but these ethanol solutions become suspensions after used several times and can not be reused. This is not only having high testing cost,resulting in a lot of waste,but also polluting the environment. The author summarized a method for recovery of waste ethanol after H. E. staining of paraffin sections,and the test proved that there was no difference in the histopathological sections produced by the gradient ethanol recovered by the method and new ethanol. The method made waste ethanol turning waste into treasure,and could extend the using life of ethanol to save the experimental funds for the laboratory while also reducing environmental pollution.
【Fund】: 国家级动物医学实验教学示范中心建设项目;; 塔里木大学动物医学专业综合改革试点项目;塔里木大学高等教育教学研究重点项目(TDGJ1310)
【CateGory Index】: S852.31
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